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Techniques for RNA in vivo imaging in plants

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JMI_2014_0134.R1.pdf (590.0Kb)
Date
04/2015
Author
Tilsner, Jens
Keywords
Lambda-N
Pumilio
Molecular beacon
MS2
RNA localisation
QH301 Biology
Metadata
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Abstract
Since the discovery of small RNAs and RNA silencing, RNA biology has taken a centre stage in cell and developmental biology. Small RNAs, but also mRNAs and other types of cellular and viral RNAs are processed at specific subcellular localizations. To fully understand cellular RNA metabolism and the various processes influenced byit, techniques are required that permit the sequence-specific tracking of RNAs in living cells. A variety of methods for RNA visualization have been developed since the 1990s, but plant cells pose particular challenges and not all approaches are applicable to them. On the other hand, plant RNA metabolism is particularly diverse and RNAs are even transported between cells, so RNA imaging can potentially provide many valuable insights into plant function at the cellular and tissue level. This Short Review briefly introduces the currently available techniques for plant RNA in vivo imaging and discusses their suitability for different biological questions.
Citation
Tilsner , J 2015 , ' Techniques for RNA in vivo imaging in plants ' , Journal of Microscopy , vol. 258 , no. 1 , pp. 1-5 . https://doi.org/10.1111/jmi.12208
Publication
Journal of Microscopy
Status
Peer reviewed
DOI
https://doi.org/10.1111/jmi.12208
ISSN
0022-2720
Type
Journal article
Rights
© 2014 The Authors. Journal of Microscopy © 2014 Royal Microscopical Society
Collections
  • University of St Andrews Research
URI
http://hdl.handle.net/10023/7929

Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

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