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Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition
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dc.contributor.author | Rollie, Clare Jane Catherine | |
dc.contributor.author | Schneider, Stefanie | |
dc.contributor.author | Brinkmann, Anna Sophie | |
dc.contributor.author | Bolt, Edward | |
dc.contributor.author | White, Malcolm F | |
dc.date.accessioned | 2015-09-21T14:10:03Z | |
dc.date.available | 2015-09-21T14:10:03Z | |
dc.date.issued | 2015-09-18 | |
dc.identifier | 210604683 | |
dc.identifier | 568a0eed-6358-4f49-9f90-a43d789b69b7 | |
dc.identifier | 84941907747 | |
dc.identifier | 000373038100001 | |
dc.identifier.citation | Rollie , C J C , Schneider , S , Brinkmann , A S , Bolt , E & White , M F 2015 , ' Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition ' , eLife , vol. 4 , e08716 . https://doi.org/10.7554/eLife.08716 | en |
dc.identifier.issn | 2050-084X | |
dc.identifier.other | ORCID: /0000-0003-1543-9342/work/47136117 | |
dc.identifier.uri | https://hdl.handle.net/10023/7519 | |
dc.description | This work was supported by a grant from the Biotechnology and Biological Sciences Research Council (REF: BB/M000400/1 to MFW). | en |
dc.description.abstract | The adaptive prokaryotic immune system CRISPR-Cas provides RNA-mediated protection from invading genetic elements. The fundamental basis of the system is the ability to capture small pieces of foreign DNA for incorporation into the genome at the CRISPR locus, a process known as Adaptation, which is dependent on the Cas1 and Cas2 proteins. We demonstrate that Cas1 catalyses an efficient trans-esterification reaction on branched DNA substrates, which represents the reverse- or disintegration reaction. Cas1 from both Escherichia coli and Sulfolobus solfataricus display sequence specific activity, with a clear preference for the nucleotides flanking the integration site at the leader-repeat 1 boundary of the CRISPR locus. Cas2 is not required for this activity and does not influence the specificity. This suggests that the inherent sequence specificity of Cas1 is a major determinant of the adaptation process. | |
dc.format.extent | 19 | |
dc.format.extent | 8816839 | |
dc.language.iso | eng | |
dc.relation.ispartof | eLife | en |
dc.subject | QH301 Biology | en |
dc.subject | BDC | en |
dc.subject | R2C | en |
dc.subject.lcc | QH301 | en |
dc.title | Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition | en |
dc.type | Journal article | en |
dc.contributor.sponsor | BBSRC | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | https://doi.org/10.7554/eLife.08716 | |
dc.description.status | Peer reviewed | en |
dc.identifier.grantnumber | BB/M000400/1 | en |
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