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dc.contributor.authorCruz, Andreia
dc.contributor.authorRodrigues, Raquel
dc.contributor.authorMonsanto Pinheiro, Miguel
dc.contributor.authorMendo, Sónia
dc.date.accessioned2015-07-21T16:10:02Z
dc.date.available2015-07-21T16:10:02Z
dc.date.issued2015-08
dc.identifier.citationCruz , A , Rodrigues , R , Monsanto Pinheiro , M & Mendo , S 2015 , ' Transcriptomes analysis of Aeromonas molluscorum Av27 cells exposed to tributyltin (TBT) : unravelling the effects from the molecular level to the organism ' , Marine Environmental Research , vol. 109 , pp. 132-139 . https://doi.org/10.1016/j.marenvres.2015.06.017en
dc.identifier.issn0141-1136
dc.identifier.otherPURE: 200632321
dc.identifier.otherPURE UUID: 1e3b880c-9a15-478f-9247-6c7622083234
dc.identifier.otherRIS: urn:A4EC2BD3752A4481F8E4A6B87186B44B
dc.identifier.otherScopus: 84939447945
dc.identifier.otherWOS: 000360514300013
dc.identifier.urihttp://hdl.handle.net/10023/7005
dc.descriptionThis work was supported by FCOMP-01-0124-FEDER-010630 project, co-funded by FEDER, European Funds through COMPETE and FCT within project PEst-C/MAR/LA0017/2013. Andreia Cruz was funded by the PhD grant SFRH/BD/36047/2007 from FCT and by a post-doctoral grant (BPD/UI88/2886/2013), from the project “Sustainable Use of Marine Resources” - MARES (CENTRO-07-ST24-FEDER-002033), funded by QREN, Mais Centro - Programa Operacional Regional do Centro e UE/Fundo Europeu de Desenvolvimento Regional. Bioinformatics support was provided by Miguel Pinheiro from the Bioinformatics Unit at St Andrews University funded by a Wellcome Trust ISSF grant (097831/Z/11/Z).en
dc.description.abstractAeromonas molluscorum Av27 cells were exposed to 0, 5 and 50 μM of TBT and the respective transcriptomes were obtained by pyrosequencing. Gene Ontology revealed that exposure to 5 μM TBT results in a higher number of repressed genes in contrast with 50 μM of TBT, where the number of over-expressed genes is greater. At both TBT concentrations, higher variations in gene expression were found in the functional categories associated with enzymatic activities, transport/binding and oxidation-reduction. A number of proteins are affected by TBT, such as the acriflavin resistance protein, several transcription-related proteins, several Hsps, ABC transporters, CorA and ZntB and other outer membrane efflux proteins, all of these involved in cellular metabolic processes, important to maintain overall cell viability. Using the STRING tool, several proteins with unknown function were related with others involved in degradation processes, such as the pyoverdine chromophore biosynthetic protein, that has been described as playing a role in the Sn-C cleavage of organotins. This approach has allowed a better understanding of the molecular effects of exposure of bacterial cells to TBT. Furthermore it contributes to the knowledge of the functional genomic aspects of bacteria exposed to this pollutant. Furthermore, the transcriptomic data gathered, and now publically available, constitute a valuable resource for comparative genome analysis.
dc.language.isoeng
dc.relation.ispartofMarine Environmental Researchen
dc.rights© 2015 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).en
dc.subjectTributyltinen
dc.subjectQuantitative real-time PCRen
dc.subjectPyoverdineen
dc.subjectDioxygenaseen
dc.subjectAcriflavineen
dc.subjectQR Microbiologyen
dc.subjectDASen
dc.subject.lccQRen
dc.titleTranscriptomes analysis of Aeromonas molluscorum Av27 cells exposed to tributyltin (TBT) : unravelling the effects from the molecular level to the organismen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Medicineen
dc.contributor.institutionUniversity of St Andrews.Gillespie Groupen
dc.identifier.doihttps://doi.org/10.1016/j.marenvres.2015.06.017
dc.description.statusPeer revieweden
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0141113615300040#appd001en


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