St Andrews Research Repository

St Andrews University Home
View Item 
  •   St Andrews Research Repository
  • University of St Andrews Research
  • University of St Andrews Research
  • University of St Andrews Research
  • View Item
  •   St Andrews Research Repository
  • University of St Andrews Research
  • University of St Andrews Research
  • University of St Andrews Research
  • View Item
  •   St Andrews Research Repository
  • University of St Andrews Research
  • University of St Andrews Research
  • University of St Andrews Research
  • View Item
  • Login
JavaScript is disabled for your browser. Some features of this site may not work without it.

Transcriptomes analysis of Aeromonas molluscorum Av27 cells exposed to tributyltin (TBT) : unravelling the effects from the molecular level to the organism

Thumbnail
View/Open
Pinheiro_2015_MER_Transcriptomes_CC.pdf (2.059Mb)
Date
08/2015
Author
Cruz, Andreia
Rodrigues, Raquel
Monsanto Pinheiro, Miguel
Mendo, Sónia
Keywords
Tributyltin
Quantitative real-time PCR
Pyoverdine
Dioxygenase
Acriflavine
QR Microbiology
DAS
Metadata
Show full item record
Altmetrics Handle Statistics
Altmetrics DOI Statistics
Abstract
Aeromonas molluscorum Av27 cells were exposed to 0, 5 and 50 μM of TBT and the respective transcriptomes were obtained by pyrosequencing. Gene Ontology revealed that exposure to 5 μM TBT results in a higher number of repressed genes in contrast with 50 μM of TBT, where the number of over-expressed genes is greater. At both TBT concentrations, higher variations in gene expression were found in the functional categories associated with enzymatic activities, transport/binding and oxidation-reduction. A number of proteins are affected by TBT, such as the acriflavin resistance protein, several transcription-related proteins, several Hsps, ABC transporters, CorA and ZntB and other outer membrane efflux proteins, all of these involved in cellular metabolic processes, important to maintain overall cell viability. Using the STRING tool, several proteins with unknown function were related with others involved in degradation processes, such as the pyoverdine chromophore biosynthetic protein, that has been described as playing a role in the Sn-C cleavage of organotins. This approach has allowed a better understanding of the molecular effects of exposure of bacterial cells to TBT. Furthermore it contributes to the knowledge of the functional genomic aspects of bacteria exposed to this pollutant. Furthermore, the transcriptomic data gathered, and now publically available, constitute a valuable resource for comparative genome analysis.
Citation
Cruz , A , Rodrigues , R , Monsanto Pinheiro , M & Mendo , S 2015 , ' Transcriptomes analysis of Aeromonas molluscorum Av27 cells exposed to tributyltin (TBT) : unravelling the effects from the molecular level to the organism ' , Marine Environmental Research , vol. 109 , pp. 132-139 . https://doi.org/10.1016/j.marenvres.2015.06.017
Publication
Marine Environmental Research
Status
Peer reviewed
DOI
https://doi.org/10.1016/j.marenvres.2015.06.017
ISSN
0141-1136
Type
Journal article
Rights
© 2015 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Description
This work was supported by FCOMP-01-0124-FEDER-010630 project, co-funded by FEDER, European Funds through COMPETE and FCT within project PEst-C/MAR/LA0017/2013. Andreia Cruz was funded by the PhD grant SFRH/BD/36047/2007 from FCT and by a post-doctoral grant (BPD/UI88/2886/2013), from the project “Sustainable Use of Marine Resources” - MARES (CENTRO-07-ST24-FEDER-002033), funded by QREN, Mais Centro - Programa Operacional Regional do Centro e UE/Fundo Europeu de Desenvolvimento Regional. Bioinformatics support was provided by Miguel Pinheiro from the Bioinformatics Unit at St Andrews University funded by a Wellcome Trust ISSF grant (097831/Z/11/Z).
Collections
  • University of St Andrews Research
URL
http://www.sciencedirect.com/science/article/pii/S0141113615300040#appd001
URI
http://hdl.handle.net/10023/7005

Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

Advanced Search

Browse

All of RepositoryCommunities & CollectionsBy Issue DateNamesTitlesSubjectsClassificationTypeFunderThis CollectionBy Issue DateNamesTitlesSubjectsClassificationTypeFunder

My Account

Login

Open Access

To find out how you can benefit from open access to research, see our library web pages and Open Access blog. For open access help contact: openaccess@st-andrews.ac.uk.

Accessibility

Read our Accessibility statement.

How to submit research papers

The full text of research papers can be submitted to the repository via Pure, the University's research information system. For help see our guide: How to deposit in Pure.

Electronic thesis deposit

Help with deposit.

Repository help

For repository help contact: Digital-Repository@st-andrews.ac.uk.

Give Feedback

Cookie policy

This site may use cookies. Please see Terms and Conditions.

Usage statistics

COUNTER-compliant statistics on downloads from the repository are available from the IRUS-UK Service. Contact us for information.

© University of St Andrews Library

University of St Andrews is a charity registered in Scotland, No SC013532.

  • Facebook
  • Twitter