Simultaneous intracellular redox potential and pH measurements in live cells using SERS nanosensors
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Intracellular redox potential is a highly regulated cellular characteristic and is critically involved in maintaining cellular health and function. The dysregulation of redox potential can result in the initiation and progression of numerous diseases. Redox potential is determined by the balance of oxidants and reductants in the cell and also by pH. For this reason a technique for quantitative measurement of intracellular redox potential and pH is highly desirable. In this paper we demonstrate how surface enhanced Raman scattering (SERS) nanosensors can be used for multiplexed measurement of both pH and redox potential in live single cells.
Jamieson , L E , Jaworska , A , Jiang , J , Baranska , M , Harrison , D J & Campbell , C J 2015 , ' Simultaneous intracellular redox potential and pH measurements in live cells using SERS nanosensors ' Analyst , vol 140 , no. 7 , pp. 2330-2335 . DOI: 10.1039/c4an02365j
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The authors gratefully acknowledge the School of Chemistry at the University of Edinburgh, a Neil Campbell Travel Award, the Faculty of Chemistry at Jagiellonian University and Jagiellonian Centre for Experimental Therapeutics (JCET). A. J.’s work was supported by National Center of Science (grant PRELUDIUM DEC-2012/05/N/ST4/00218) and by the European Union from the resources of the European Regional Development Fund under the Innovative Economy Programme (grant coordinated by JCET-UJ, No POIG.01.01.02-00-069/09).
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