Show simple item record

Files in this item

Thumbnail

Item metadata

dc.contributor.authordi Gesso, J.L.
dc.contributor.authorKerr, J.S.
dc.contributor.authorZhang, Q.
dc.contributor.authorRaheem, S.
dc.contributor.authorYalamanchili, S.K.
dc.contributor.authorO'Hagan, D.
dc.contributor.authorKay, C.D.
dc.contributor.authorO'Connell, M.A.
dc.date.accessioned2015-05-28T16:10:08Z
dc.date.available2015-05-28T16:10:08Z
dc.date.issued2015-06
dc.identifier.citationdi Gesso , J L , Kerr , J S , Zhang , Q , Raheem , S , Yalamanchili , S K , O'Hagan , D , Kay , C D & O'Connell , M A 2015 , ' Flavonoid metabolites reduce tumor necrosis factor-α secretion to a greater extent than their precursor compounds in human THP-1 monocytes ' , Molecular Nutrition and Food Research , vol. 59 , no. 6 , pp. 1143-1154 . https://doi.org/10.1002/mnfr.201400799en
dc.identifier.issn1613-4125
dc.identifier.otherPURE: 191127476
dc.identifier.otherPURE UUID: 5464dac4-4465-43de-a430-8eefad0e7f8c
dc.identifier.otherScopus: 84930177527
dc.identifier.otherWOS: 000355745400012
dc.identifier.otherORCID: /0000-0002-0510-5552/work/68281213
dc.identifier.urihttps://hdl.handle.net/10023/6709
dc.descriptionThis study was supported by funding from the UK Biotechnology and Biological Sciences Research Council Diet and Health Research Industry Club (BBSRC-DRINC) (BB/I006028/1) and by a studentship grant (BB/J500100/1).en
dc.description.abstractScope: Flavonoids are generally studied in vitro, in isolation, and as unmetabolized precursor structures. However, in the habitual diet, multiple flavonoids are consumed together and found present in the circulation as complex mixtures of metabolites. Using a unique study design, we investigated the potential for singular or additive anti-inflammatory effects of flavonoid metabolites relative to their precursor structures. Methods and results: Six flavonoids, 14 flavonoid metabolites, and 29 combinations of flavonoids and their metabolites (0.1-10 μM) were screened for their ability to reduce LPS-induced tumor necrosis factor-α (TNF-α) secretion in THP-1 monocytes. One micromolar peonidin-3-glucoside, cyanidin-3-glucoside, and the metabolites isovanillic acid (IVA), IVA-glucuronide, vanillic acid-glucuronide, protocatechuic acid-3-sulfate, and benzoic acid-sulfate significantly reduced TNF-α secretion when in isolation, while there was no effect on TNF-α mRNA expression. Four combinations of metabolites that included 4-hydroxybenzoic acid (4HBA) and/or protocatechuic acid also significantly reduced TNF-α secretion to a greater extent than the precursors or metabolites alone. The effects on LPS-induced IL-1β and IL-10 secretion and mRNA expression were also examined. 4HBA significantly reduced IL-1β secretion but none of the flavonoids or metabolites significantly modified IL-10 secretion. Conclusion: This study provides novel evidence suggesting flavonoid bioactivity results from cumulative or additive effects of circulating metabolites.
dc.language.isoeng
dc.relation.ispartofMolecular Nutrition and Food Researchen
dc.rights© 2015 The Authors. Molecular Nutrition & Food Research published by Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en
dc.subjectCytokineen
dc.subjectInflammationen
dc.subjectMetabolismen
dc.subjectPhase 2 conjugatesen
dc.subjectPolyphenolen
dc.subjectQD Chemistryen
dc.subjectNDASen
dc.subject.lccQDen
dc.titleFlavonoid metabolites reduce tumor necrosis factor-α secretion to a greater extent than their precursor compounds in human THP-1 monocytesen
dc.typeJournal articleen
dc.contributor.sponsorBBSRCen
dc.contributor.sponsorBBSRCen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Chemistryen
dc.contributor.institutionUniversity of St Andrews. EaSTCHEMen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1002/mnfr.201400799
dc.description.statusPeer revieweden
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1002/mnfr.201400799/suppinfoen
dc.identifier.grantnumberBB/H004726/1en
dc.identifier.grantnumberBB/I005943/1en


This item appears in the following Collection(s)

Show simple item record