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Flavonoid metabolites reduce tumor necrosis factor-α secretion to a greater extent than their precursor compounds in human THP-1 monocytes

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O_Hagan_2015_MNRS_Flavonoid_CC.pdf (471.9Kb)
Date
06/2015
Author
di Gesso, J.L.
Kerr, J.S.
Zhang, Q.
Raheem, S.
Yalamanchili, S.K.
O'Hagan, D.
Kay, C.D.
O'Connell, M.A.
Funder
BBSRC
BBSRC
Grant ID
BB/H004726/1
BB/I005943/1
Keywords
Cytokine
Inflammation
Metabolism
Phase 2 conjugates
Polyphenol
QD Chemistry
NDAS
Metadata
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Abstract
Scope: Flavonoids are generally studied in vitro, in isolation, and as unmetabolized precursor structures. However, in the habitual diet, multiple flavonoids are consumed together and found present in the circulation as complex mixtures of metabolites. Using a unique study design, we investigated the potential for singular or additive anti-inflammatory effects of flavonoid metabolites relative to their precursor structures. Methods and results: Six flavonoids, 14 flavonoid metabolites, and 29 combinations of flavonoids and their metabolites (0.1-10 μM) were screened for their ability to reduce LPS-induced tumor necrosis factor-α (TNF-α) secretion in THP-1 monocytes. One micromolar peonidin-3-glucoside, cyanidin-3-glucoside, and the metabolites isovanillic acid (IVA), IVA-glucuronide, vanillic acid-glucuronide, protocatechuic acid-3-sulfate, and benzoic acid-sulfate significantly reduced TNF-α secretion when in isolation, while there was no effect on TNF-α mRNA expression. Four combinations of metabolites that included 4-hydroxybenzoic acid (4HBA) and/or protocatechuic acid also significantly reduced TNF-α secretion to a greater extent than the precursors or metabolites alone. The effects on LPS-induced IL-1β and IL-10 secretion and mRNA expression were also examined. 4HBA significantly reduced IL-1β secretion but none of the flavonoids or metabolites significantly modified IL-10 secretion. Conclusion: This study provides novel evidence suggesting flavonoid bioactivity results from cumulative or additive effects of circulating metabolites.
Citation
di Gesso , J L , Kerr , J S , Zhang , Q , Raheem , S , Yalamanchili , S K , O'Hagan , D , Kay , C D & O'Connell , M A 2015 , ' Flavonoid metabolites reduce tumor necrosis factor-α secretion to a greater extent than their precursor compounds in human THP-1 monocytes ' , Molecular Nutrition and Food Research , vol. 59 , no. 6 , pp. 1143-1154 . https://doi.org/10.1002/mnfr.201400799
Publication
Molecular Nutrition and Food Research
Status
Peer reviewed
DOI
https://doi.org/10.1002/mnfr.201400799
ISSN
1613-4125
Type
Journal article
Rights
© 2015 The Authors. Molecular Nutrition & Food Research published by Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Description
This study was supported by funding from the UK Biotechnology and Biological Sciences Research Council Diet and Health Research Industry Club (BBSRC-DRINC) (BB/I006028/1) and by a studentship grant (BB/J500100/1).
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  • University of St Andrews Research
URL
http://onlinelibrary.wiley.com/doi/10.1002/mnfr.201400799/suppinfo
URI
http://hdl.handle.net/10023/6709

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