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dc.contributor.authorNylk, Jonathan
dc.contributor.authorKristensen, M. V. G.
dc.contributor.authorMazilu, Michael
dc.contributor.authorKubasik-Thayil, Anisha
dc.contributor.authorMitchell, C. A.
dc.contributor.authorCampbell, Elaine Catherine
dc.contributor.authorPowis, Simon John
dc.contributor.authorGunn-Moore, Frank J
dc.contributor.authorDholakia, Kishan
dc.date.accessioned2015-04-06T14:31:02Z
dc.date.available2015-04-06T14:31:02Z
dc.date.issued2015-04-01
dc.identifier175276399
dc.identifier9e9c0ea7-3162-46da-b47f-bd819798ac2a
dc.identifier84948771934
dc.identifier000352228400036
dc.identifier.citationNylk , J , Kristensen , M V G , Mazilu , M , Kubasik-Thayil , A , Mitchell , C A , Campbell , E C , Powis , S J , Gunn-Moore , F J & Dholakia , K 2015 , ' Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis ' , Biomedical Optics Express , vol. 6 , no. 4 , pp. 1512-1519 . https://doi.org/10.1364/BOE.6.001512en
dc.identifier.issn2156-7085
dc.identifier.otherORCID: /0000-0003-3422-3387/work/34730422
dc.identifier.otherORCID: /0000-0002-2977-4929/work/33493305
dc.identifier.otherORCID: /0000-0003-4218-2984/work/60195313
dc.identifier.urihttps://hdl.handle.net/10023/6435
dc.descriptionThe authors thank the UK Engineering and Physical Sciences Research Council under grant EP/J01771X/1.en
dc.description.abstractWe demonstrate a miniaturized single beam fiber optical trapping probe based on a high numerical aperture graded index (GRIN) micro-objective lens. This enables optical trapping at a distance of 200μm from the probe tip. The fiber trapping probe is characterized experimentally using power spectral density analysis and an original approach based on principal component analysis for accurate particle tracking. Its use for biomedical microscopy is demonstrated through optically mediated immunological synapse formation.
dc.format.extent607478
dc.language.isoeng
dc.relation.ispartofBiomedical Optics Expressen
dc.subjectQC Physicsen
dc.subjectNDASen
dc.subject.lccQCen
dc.titleDevelopment of a graded index microlens based fiber optical trap and its characterization using principal component analysisen
dc.typeJournal articleen
dc.contributor.sponsorEPSRCen
dc.contributor.institutionUniversity of St Andrews. School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Institute of Behavioural and Neural Sciencesen
dc.identifier.doi10.1364/BOE.6.001512
dc.description.statusPeer revieweden
dc.identifier.grantnumberEP/J01771X/1en


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