Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis
Abstract
We demonstrate a miniaturized single beam fiber optical trapping probe based on a high numerical aperture graded index (GRIN) micro-objective lens. This enables optical trapping at a distance of 200μm from the probe tip. The fiber trapping probe is characterized experimentally using power spectral density analysis and an original approach based on principal component analysis for accurate particle tracking. Its use for biomedical microscopy is demonstrated through optically mediated immunological synapse formation.
Citation
Nylk , J , Kristensen , M V G , Mazilu , M , Kubasik-Thayil , A , Mitchell , C A , Campbell , E C , Powis , S J , Gunn-Moore , F J & Dholakia , K 2015 , ' Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis ' , Biomedical Optics Express , vol. 6 , no. 4 , pp. 1512-1519 . https://doi.org/10.1364/BOE.6.001512
Publication
Biomedical Optics Express
Status
Peer reviewed
ISSN
2156-7085Type
Journal article
Rights
© 2015 Optical Society of America. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited
Description
The authors thank the UK Engineering and Physical Sciences Research Council under grant EP/J01771X/1.Collections
Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.