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Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis

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Nylk_2015_BOE_Dev_AM.pdf (593.2Kb)
Date
01/04/2015
Author
Nylk, Jonathan
Kristensen, M. V. G.
Mazilu, Michael
Kubasik-Thayil, Anisha
Mitchell, C. A.
Campbell, Elaine Catherine
Powis, Simon John
Gunn-Moore, Frank J
Dholakia, Kishan
Keywords
QC Physics
NDAS
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Abstract
We demonstrate a miniaturized single beam fiber optical trapping probe based on a high numerical aperture graded index (GRIN) micro-objective lens. This enables optical trapping at a distance of 200μm from the probe tip. The fiber trapping probe is characterized experimentally using power spectral density analysis and an original approach based on principal component analysis for accurate particle tracking. Its use for biomedical microscopy is demonstrated through optically mediated immunological synapse formation.
Citation
Nylk , J , Kristensen , M V G , Mazilu , M , Kubasik-Thayil , A , Mitchell , C A , Campbell , E C , Powis , S J , Gunn-Moore , F J & Dholakia , K 2015 , ' Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis ' , Biomedical Optics Express , vol. 6 , no. 4 , pp. 1512-1519 . https://doi.org/10.1364/BOE.6.001512
Publication
Biomedical Optics Express
Status
Peer reviewed
DOI
https://doi.org/10.1364/BOE.6.001512
ISSN
2156-7085
Type
Journal article
Rights
© 2015 Optical Society of America. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited
Description
The authors thank the UK Engineering and Physical Sciences Research Council under grant EP/J01771X/1.
Collections
  • University of St Andrews Research
URI
http://hdl.handle.net/10023/6435

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