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dc.contributor.authorPlowman, Sarah J
dc.contributor.authorWilliamson, D James
dc.contributor.authorO'Sullivan, Maureen J
dc.contributor.authorDoig, Jennifer
dc.contributor.authorRitchie, Ann-Marie
dc.contributor.authorHarrison, David J
dc.contributor.authorMelton, David W
dc.contributor.authorArends, Mark J
dc.contributor.authorHooper, Martin L
dc.contributor.authorPatek, Charles E
dc.identifier.citationPlowman , S J , Williamson , D J , O'Sullivan , M J , Doig , J , Ritchie , A-M , Harrison , D J , Melton , D W , Arends , M J , Hooper , M L & Patek , C E 2003 , ' While K-ras is essential for mouse development, expression of the K-ras 4A splice variant is dispensable ' , Molecular and Cellular Biology , vol. 23 , no. 24 , pp. 9245-9250 .
dc.identifier.otherPURE: 173403505
dc.identifier.otherPURE UUID: d6fc3ec4-8d02-4feb-b874-381866cc8e30
dc.identifier.otherRIS: urn:B36DE42E2137513F7F973D8B6B5676EE
dc.identifier.otherScopus: 10744231278
dc.identifier.otherORCID: /0000-0001-9041-9988/work/64034278
dc.description.abstractIn mammals, the three classical ras genes encode four highly homologous proteins, N-Ras, H-Ras, and the isoforms K-Ras 4A and 4B. Previous studies have shown that K-ras is essential for mouse development and that while K-ras 4A and 4B are expressed during development, K-ras 4A expression is regulated temporally and spatially and occurs in adult kidney, intestine, stomach, and liver. In the present study, the pattern of K-ras 4A expression was examined in a wide range of wild-type adult mouse tissues, and gene targeting was used to generate K-ras 4A-deficient mice to examine its role in development. It was found that K-ras 4A is also expressed in uterus, lung, pancreas, salivary glands, seminal vesicles, bone marrow cells, and cecum, where it was the major K-Ras isoform expressed. Mating between K-ras(tmDelta4A/+) mice produced viable K-ras(tmDelta4A/tmDelta4A) offspring with the expected Mendelian ratios of inheritance, and these mice expressed the K-ras 4B splice variant only. K-ras(tmDelta4A/tmDelta4A) mice were fertile and showed no histopathological abnormalities on inbred (129/Ola) or crossbred (129/Ola x C57BL/6) genetic backgrounds. The results demonstrate that K-Ras 4A, like H- and N-Ras, is dispensable for normal mouse development, at least in the presence of functional K-Ras 4B.
dc.relation.ispartofMolecular and Cellular Biologyen
dc.rightsCopyright © 2003, American Society for Microbiology. All Rights Reserved.en
dc.subjectAlternative splicingen
dc.subjectBase sequenceen
dc.subjectGene expression regulation, Developmentalen
dc.subjectGenes, Rasen
dc.subjectMice, Inbred C57BLen
dc.subjectMice, knockouten
dc.subjectProtein Isoformsen
dc.subjectTissue distributionen
dc.subjectR Medicineen
dc.subjectRC0254 Neoplasms. Tumors. Oncology (including Cancer)en
dc.titleWhile K-ras is essential for mouse development, expression of the K-ras 4A splice variant is dispensableen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Medicineen
dc.description.statusPeer revieweden

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