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dc.contributor.authorLeeper, Alexander D
dc.contributor.authorFarrell, Joanne
dc.contributor.authorDixon, J Michael
dc.contributor.authorWedden, Sarah E
dc.contributor.authorHarrison, David J
dc.contributor.authorKatz, Elad
dc.identifier.citationLeeper , A D , Farrell , J , Dixon , J M , Wedden , S E , Harrison , D J & Katz , E 2011 , ' Long-term culture of human breast cancer specimens and their analysis using optical projection tomography ' , Journal of Visualized Experiments , vol. 53 , e3085 .
dc.identifier.otherPURE: 173395599
dc.identifier.otherPURE UUID: 6fbec6b3-8c4d-48a4-923f-854a24b8eb93
dc.identifier.otherRIS: urn:40D5339F4E80C63927CE4C08DDAAAAD6
dc.identifier.otherScopus: 80355132685
dc.identifier.otherORCID: /0000-0001-9041-9988/work/64034242
dc.description.abstractBreast cancer is a leading cause of mortality in the Western world. It is well established that the spread of breast cancer, first locally and later distally, is a major factor in patient prognosis. Experimental systems of breast cancer rely on cell lines usually derived from primary tumours or pleural effusions. Two major obstacles hinder this research: (i) some known sub-types of breast cancers (notably poor prognosis luminal B tumours) are not represented within current line collections; (ii) the influence of the tumour microenvironment is not usually taken into account. We demonstrate a technique to culture primary breast cancer specimens of all sub-types. This is achieved by using three-dimensional (3D) culture system in which small pieces of tumour are embedded in soft rat collagen I cushions. Within 2-3 weeks, the tumour cells spread into the collagen and form various structures similar to those observed in human tumours1. Viable adipocytes, epithelial cells and fibroblasts within the original core were evident on histology. Malignant epithelial cells with squamoid morphology were demonstrated invading into the surrounding collagen. Nuclear pleomorphism was evident within these cells, along with mitotic figures and apoptotic bodies. We have employed Optical Projection Tomography (OPT), a 3D imaging technology, in order to quantify the extent of tumour spread in culture. We have used OPT to measure the bulk volume of the tumour culture, a parameter routinely measured during the neo-adjuvant treatment of breast cancer patients to assess response to drug therapy. Here, we present an opportunity to culture human breast tumours without sub-type bias and quantify the spread of those ex vivo. This method could be used in the future to quantify drug sensitivity in original tumour. This may provide a more predictive model than currently used cell lines.
dc.relation.ispartofJournal of Visualized Experimentsen
dc.rightsCopyright © 2011 Creative Commons Attribution-NonCommercial License. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.subjectBiochemistry, Genetics and Molecular Biology(all)en
dc.subjectImmunology and Microbiology(all)en
dc.subjectChemical Engineering(all)en
dc.subjectR Medicineen
dc.subjectSDG 3 - Good Health and Well-beingen
dc.titleLong-term culture of human breast cancer specimens and their analysis using optical projection tomographyen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.description.statusPeer revieweden

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