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Specific nuclear envelope transmembrane proteins can promote the location of chromosomes to and from the nuclear periphery

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zuleger2013genomebiolR14.pdf (9.487Mb)
Date
15/02/2013
Author
Zuleger, Nikolaj
Boyle, Shelagh
Kelly, David A
de Las Heras, Jose I
Lazou, Vassiliki
Korfali, Nadia
Batrakou, Dzmitry G
Randles, K Natalie
Morris, Glenn E
Harrison, David J
Bickmore, Wendy A
Schirmer, Eric C
Keywords
Genetics
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Abstract
BACKGROUND: Different cell types have distinctive patterns of chromosome positioning in the nucleus. Although ectopic affinity-tethering of specific loci can be used to relocate chromosomes to the nuclear periphery, endogenous nuclear envelope proteins that control such a mechanism in mammalian cells have yet to be widely identified. RESULTS: To search for such proteins twenty three nuclear envelope transmembrane proteins were screened for their ability to promote peripheral localization of human chromosomes in HT1080 fibroblasts. Five of these proteins had strong effects on chromosome 5, but individual proteins affected different subsets of chromosomes. The repositioning effects were reversible and the proteins with effects all exhibited highly tissue-restricted patterns of expression. Depletion of two nuclear envelope transmembrane proteins that were preferentially expressed in liver each reduced the normal peripheral positioning of chromosome 5 in liver cells. CONCLUSIONS: The discovery of nuclear envelope transmembrane proteins that can modulate chromosome position and have restricted patterns of expression may enable dissection of the functional relevance of tissue-specific patterns of radial chromosome positioning.
Citation
Zuleger , N , Boyle , S , Kelly , D A , de Las Heras , J I , Lazou , V , Korfali , N , Batrakou , D G , Randles , K N , Morris , G E , Harrison , D J , Bickmore , W A & Schirmer , E C 2013 , ' Specific nuclear envelope transmembrane proteins can promote the location of chromosomes to and from the nuclear periphery ' , Genome Biology , vol. 14 , no. 2 , R14 . https://doi.org/10.1186/gb-2013-14-2-r14
Publication
Genome Biology
Status
Peer reviewed
DOI
https://doi.org/10.1186/gb-2013-14-2-r14
ISSN
1465-6906
Type
Journal article
Rights
© 2013 Zuleger et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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  • University of St Andrews Research
URI
http://hdl.handle.net/10023/6228

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