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dc.contributor.authorYang, Zhengyi
dc.contributor.authorProkopas, Martynas
dc.contributor.authorNylk, Jonathan
dc.contributor.authorColl Llado, Clara
dc.contributor.authorGunn-Moore, Frank J
dc.contributor.authorFerrier, David Ellard Keith
dc.contributor.authorVettenburg, Tom
dc.contributor.authorDholakia, Kishan
dc.date.accessioned2014-12-16T17:31:03Z
dc.date.available2014-12-16T17:31:03Z
dc.date.issued2014-10-01
dc.identifier.citationYang , Z , Prokopas , M , Nylk , J , Coll Llado , C , Gunn-Moore , F J , Ferrier , D E K , Vettenburg , T & Dholakia , K 2014 , ' A compact Airy beam light sheet microscope with a tilted cylindrical lens ' , Biomedical Optics Express , vol. 5 , no. 10 , pp. 3434-3442 . https://doi.org/10.1364/BOE.5.003434en
dc.identifier.issn2156-7085
dc.identifier.otherPURE: 146215895
dc.identifier.otherPURE UUID: 3f86ed90-6184-4f31-8963-5a606c168eb0
dc.identifier.otherScopus: 84942374722
dc.identifier.otherORCID: /0000-0003-3247-6233/work/36423820
dc.identifier.otherORCID: /0000-0003-3422-3387/work/34730425
dc.identifier.otherORCID: /0000-0002-2977-4929/work/33493306
dc.identifier.otherWOS: 000343135200014
dc.identifier.urihttp://hdl.handle.net/10023/5930
dc.descriptionWe thank the UK Engineering and Physical Sciences Research Council under grant EP/J01771X/1, the ’BRAINS’ 600th anniversary appeal and Dr. E. Killick for funding.en
dc.description.abstractLight-sheet imaging is rapidly gaining importance for imaging intact biological specimens. Many of the latest innovations rely on the propagation-invariant Bessel or Airy beams to form an extended light sheet to provide high resolution across a large field of view. Shaping light to realize propagation-invariant beams often relies on complex programming of spatial light modulators or specialized, custom made, optical elements. Here we present a straightforward and low-cost modification to the traditional light-sheet setup, based on the open-access light-sheet microscope OpenSPIM, to achieve Airy light-sheet illumination. This brings wide field single-photon light-sheet imaging to a broader range of endusers. Fluorescent microspheres embedded in agarose and a zebrafish larva were imaged to demonstrate how such a microscope can have a minimal footprint and cost without compromising on imaging quality.
dc.language.isoeng
dc.relation.ispartofBiomedical Optics Expressen
dc.rights(c) Copyright 2014 Optical Society of America. This paper was published in Biomedical Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: [http://www.opticsinfobase.org/boe/abstract.cfm?uri=boe-5-10-3434]. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.en
dc.subjectQC Physicsen
dc.subject.lccQCen
dc.titleA compact Airy beam light sheet microscope with a tilted cylindrical lensen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Institute of Behavioural and Neural Sciencesen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews.Marine Alliance for Science & Technology Scotlanden
dc.contributor.institutionUniversity of St Andrews.Scottish Oceans Instituteen
dc.identifier.doihttps://doi.org/10.1364/BOE.5.003434
dc.description.statusPeer revieweden


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