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A compact Airy beam light sheet microscope with a tilted cylindrical lens
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dc.contributor.author | Yang, Zhengyi | |
dc.contributor.author | Prokopas, Martynas | |
dc.contributor.author | Nylk, Jonathan | |
dc.contributor.author | Coll Llado, Clara | |
dc.contributor.author | Gunn-Moore, Frank J | |
dc.contributor.author | Ferrier, David Ellard Keith | |
dc.contributor.author | Vettenburg, Tom | |
dc.contributor.author | Dholakia, Kishan | |
dc.date.accessioned | 2014-12-16T17:31:03Z | |
dc.date.available | 2014-12-16T17:31:03Z | |
dc.date.issued | 2014-10-01 | |
dc.identifier | 146215895 | |
dc.identifier | 3f86ed90-6184-4f31-8963-5a606c168eb0 | |
dc.identifier | 84942374722 | |
dc.identifier | 000343135200014 | |
dc.identifier.citation | Yang , Z , Prokopas , M , Nylk , J , Coll Llado , C , Gunn-Moore , F J , Ferrier , D E K , Vettenburg , T & Dholakia , K 2014 , ' A compact Airy beam light sheet microscope with a tilted cylindrical lens ' , Biomedical Optics Express , vol. 5 , no. 10 , pp. 3434-3442 . https://doi.org/10.1364/BOE.5.003434 | en |
dc.identifier.issn | 2156-7085 | |
dc.identifier.other | ORCID: /0000-0003-3247-6233/work/36423820 | |
dc.identifier.other | ORCID: /0000-0003-3422-3387/work/34730425 | |
dc.identifier.other | ORCID: /0000-0002-2977-4929/work/33493306 | |
dc.identifier.uri | https://hdl.handle.net/10023/5930 | |
dc.description | We thank the UK Engineering and Physical Sciences Research Council under grant EP/J01771X/1, the ’BRAINS’ 600th anniversary appeal and Dr. E. Killick for funding. | en |
dc.description.abstract | Light-sheet imaging is rapidly gaining importance for imaging intact biological specimens. Many of the latest innovations rely on the propagation-invariant Bessel or Airy beams to form an extended light sheet to provide high resolution across a large field of view. Shaping light to realize propagation-invariant beams often relies on complex programming of spatial light modulators or specialized, custom made, optical elements. Here we present a straightforward and low-cost modification to the traditional light-sheet setup, based on the open-access light-sheet microscope OpenSPIM, to achieve Airy light-sheet illumination. This brings wide field single-photon light-sheet imaging to a broader range of endusers. Fluorescent microspheres embedded in agarose and a zebrafish larva were imaged to demonstrate how such a microscope can have a minimal footprint and cost without compromising on imaging quality. | |
dc.format.extent | 2515235 | |
dc.language.iso | eng | |
dc.relation.ispartof | Biomedical Optics Express | en |
dc.subject | QC Physics | en |
dc.subject.lcc | QC | en |
dc.title | A compact Airy beam light sheet microscope with a tilted cylindrical lens | en |
dc.type | Journal article | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.institution | University of St Andrews. School of Physics and Astronomy | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.contributor.institution | University of St Andrews. Institute of Behavioural and Neural Sciences | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.contributor.institution | University of St Andrews. Marine Alliance for Science & Technology Scotland | en |
dc.contributor.institution | University of St Andrews. Scottish Oceans Institute | en |
dc.identifier.doi | 10.1364/BOE.5.003434 | |
dc.description.status | Peer reviewed | en |
dc.identifier.grantnumber | EP/J01771X/1 | en |
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