A compact Airy beam light sheet microscope with a tilted cylindrical lens
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Light-sheet imaging is rapidly gaining importance for imaging intact biological specimens. Many of the latest innovations rely on the propagation-invariant Bessel or Airy beams to form an extended light sheet to provide high resolution across a large field of view. Shaping light to realize propagation-invariant beams often relies on complex programming of spatial light modulators or specialized, custom made, optical elements. Here we present a straightforward and low-cost modification to the traditional light-sheet setup, based on the open-access light-sheet microscope OpenSPIM, to achieve Airy light-sheet illumination. This brings wide field single-photon light-sheet imaging to a broader range of endusers. Fluorescent microspheres embedded in agarose and a zebrafish larva were imaged to demonstrate how such a microscope can have a minimal footprint and cost without compromising on imaging quality.
Yang , Z , Prokopas , M , Nylk , J , Coll Llado , C , Gunn-Moore , F J , Ferrier , D E K , Vettenburg , T & Dholakia , K 2014 , ' A compact Airy beam light sheet microscope with a tilted cylindrical lens ' Biomedical Optics Express , vol. 5 , no. 10 , pp. 3434-3442 . https://doi.org/10.1364/BOE.5.003434
Biomedical Optics Express
(c) Copyright 2014 Optical Society of America. This paper was published in Biomedical Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: [http://www.opticsinfobase.org/boe/abstract.cfm?uri=boe-5-10-3434]. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.
DescriptionWe thank the UK Engineering and Physical Sciences Research Council under grant EP/J01771X/1, the ’BRAINS’ 600th anniversary appeal and Dr. E. Killick for funding.
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