A compact Airy beam light sheet microscope with a tilted cylindrical lens
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Date
01/10/2014Author
Funder
Grant ID
EP/J01771X/1
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Show full item recordAbstract
Light-sheet imaging is rapidly gaining importance for imaging intact biological specimens. Many of the latest innovations rely on the propagation-invariant Bessel or Airy beams to form an extended light sheet to provide high resolution across a large field of view. Shaping light to realize propagation-invariant beams often relies on complex programming of spatial light modulators or specialized, custom made, optical elements. Here we present a straightforward and low-cost modification to the traditional light-sheet setup, based on the open-access light-sheet microscope OpenSPIM, to achieve Airy light-sheet illumination. This brings wide field single-photon light-sheet imaging to a broader range of endusers. Fluorescent microspheres embedded in agarose and a zebrafish larva were imaged to demonstrate how such a microscope can have a minimal footprint and cost without compromising on imaging quality.
Citation
Yang , Z , Prokopas , M , Nylk , J , Coll Llado , C , Gunn-Moore , F J , Ferrier , D E K , Vettenburg , T & Dholakia , K 2014 , ' A compact Airy beam light sheet microscope with a tilted cylindrical lens ' , Biomedical Optics Express , vol. 5 , no. 10 , pp. 3434-3442 . https://doi.org/10.1364/BOE.5.003434
Publication
Biomedical Optics Express
Status
Peer reviewed
ISSN
2156-7085Type
Journal article
Description
We thank the UK Engineering and Physical Sciences Research Council under grant EP/J01771X/1, the ’BRAINS’ 600th anniversary appeal and Dr. E. Killick for funding.Collections
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