Strategies for synthesis of yardsticks and abaci for nanometre distance measurements by pulsed EPR
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Pulsed electron paramagnetic resonance (EPR) techniques have been found to be an efficient tool for elucidation of structure in complex biological systems as they give access to distances in the nanometre range. These measurements can provide additional structural information such as relative orientations, structural flexibility or aggregation states. A wide variety of model systems for calibration and optimisation of pulsed experiments has been synthesised. Their design is based on mimicking biological systems or materials in specific properties such as the distances themselves and the distance distributions. Here, we review selected approaches to the synthesis of chemical systems bearing two or more spin centres, such as nitroxide or trityl radicals, metal ions or combinations thereof and sketch their application in pulsed EPR distance measurements.
Valera , S & Bode , B E 2014 , ' Strategies for synthesis of yardsticks and abaci for nanometre distance measurements by pulsed EPR ' Molecules , vol 19 , no. 12 , pp. 20227-20256 . DOI: 10.3390/molecules191220227
© 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
Silvia Valera is grateful for support by EPSRC and Bela E. Bode acknowledges support by EastCHEM.
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