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Light-sheet microscopy using an Airy beam
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dc.contributor.author | Vettenburg, Tom | |
dc.contributor.author | Dalgarno, Heather Isla Campbell | |
dc.contributor.author | Nylk, Jonathan | |
dc.contributor.author | Coll-Lladó, Clara | |
dc.contributor.author | Ferrier, David Ellard Keith | |
dc.contributor.author | Čižmár, Tomáš | |
dc.contributor.author | Gunn-Moore, Frank J | |
dc.contributor.author | Dholakia, Kishan | |
dc.date.accessioned | 2014-09-30T23:01:59Z | |
dc.date.available | 2014-09-30T23:01:59Z | |
dc.date.issued | 2014-05 | |
dc.identifier | 108677751 | |
dc.identifier | fc3f9dd0-1d4f-4edb-80dc-dfb070ec06c1 | |
dc.identifier | 84899971983 | |
dc.identifier | 000335873400019 | |
dc.identifier.citation | Vettenburg , T , Dalgarno , H I C , Nylk , J , Coll-Lladó , C , Ferrier , D E K , Čižmár , T , Gunn-Moore , F J & Dholakia , K 2014 , ' Light-sheet microscopy using an Airy beam ' , Nature Methods , vol. 11 , no. 5 , pp. 541-544 . https://doi.org/10.1038/nmeth.2922 | en |
dc.identifier.issn | 1548-7091 | |
dc.identifier.other | ORCID: /0000-0003-3247-6233/work/36423822 | |
dc.identifier.other | ORCID: /0000-0003-3422-3387/work/34730428 | |
dc.identifier.other | ORCID: /0000-0002-2977-4929/work/33493307 | |
dc.identifier.uri | https://hdl.handle.net/10023/5521 | |
dc.description | The project was supported by the UK Engineering and Physical Sciences Research Council, RS Macdonald Charitable Trust, Scottish Universities Life Sciences Alliance and St Andrews 600 anniversary BRAINS appeal. The PhD apprenticeship of C.C.L. was funded by the School of Biology. | en |
dc.description.abstract | Light-sheet microscopy facilitates rapid, high-contrast, volumetric imaging with minimal sample exposure. However, the rapid divergence of a traditional Gaussian light sheet restricts the field of view (FOV) that provides innate subcellular resolution. We show that the Airy beam innately yields high contrast and resolution up to a tenfold larger FOV. In contrast to the Bessel beam, which also provides an increased FOV, the Airy beam's characteristic asymmetric excitation pattern results in all fluorescence contributing positively to the contrast, enabling a step change for light-sheet microscopy. | |
dc.format.extent | 7 | |
dc.format.extent | 5525944 | |
dc.language.iso | eng | |
dc.relation.ispartof | Nature Methods | en |
dc.subject | QC Physics | en |
dc.subject | BDC | en |
dc.subject | R2C | en |
dc.subject.lcc | QC | en |
dc.title | Light-sheet microscopy using an Airy beam | en |
dc.type | Journal article | en |
dc.contributor.institution | University of St Andrews. School of Physics and Astronomy | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.contributor.institution | University of St Andrews. Marine Alliance for Science & Technology Scotland | en |
dc.contributor.institution | University of St Andrews. Scottish Oceans Institute | en |
dc.contributor.institution | University of St Andrews. School of Medicine | en |
dc.contributor.institution | University of St Andrews. Institute of Behavioural and Neural Sciences | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | 10.1038/nmeth.2922 | |
dc.description.status | Peer reviewed | en |
dc.date.embargoedUntil | 2014-10-01 |
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