St Andrews Research Repository

St Andrews University Home
View Item 
  •   St Andrews Research Repository
  • University of St Andrews Research
  • University of St Andrews Research
  • University of St Andrews Research
  • View Item
  •   St Andrews Research Repository
  • University of St Andrews Research
  • University of St Andrews Research
  • University of St Andrews Research
  • View Item
  •   St Andrews Research Repository
  • University of St Andrews Research
  • University of St Andrews Research
  • University of St Andrews Research
  • View Item
  • Register / Login
JavaScript is disabled for your browser. Some features of this site may not work without it.

Light-sheet microscopy using an Airy beam

Thumbnail
View/Open
airsheet_preproof.pdf (5.269Mb)
Date
05/2014
Author
Vettenburg, Tom
Dalgarno, Heather Isla Campbell
Nylk, Jonathan
Coll-Lladó, Clara
Ferrier, David Ellard Keith
Čižmár, Tomáš
Gunn-Moore, Frank J
Dholakia, Kishan
Keywords
QC Physics
BDC
R2C
Metadata
Show full item record
Abstract
Light-sheet microscopy facilitates rapid, high-contrast, volumetric imaging with minimal sample exposure. However, the rapid divergence of a traditional Gaussian light sheet restricts the field of view (FOV) that provides innate subcellular resolution. We show that the Airy beam innately yields high contrast and resolution up to a tenfold larger FOV. In contrast to the Bessel beam, which also provides an increased FOV, the Airy beam's characteristic asymmetric excitation pattern results in all fluorescence contributing positively to the contrast, enabling a step change for light-sheet microscopy.
Citation
Vettenburg , T , Dalgarno , H I C , Nylk , J , Coll-Lladó , C , Ferrier , D E K , Čižmár , T , Gunn-Moore , F J & Dholakia , K 2014 , ' Light-sheet microscopy using an Airy beam ' , Nature Methods , vol. 11 , no. 5 , pp. 541-544 . https://doi.org/10.1038/nmeth.2922
Publication
Nature Methods
Status
Peer reviewed
DOI
https://doi.org/10.1038/nmeth.2922
ISSN
1548-7091
Type
Journal article
Rights
© 2014 Macmillan Publishers Limited. All Rights Reserved. Users may view, print, copy, download and text and data-mine the content, for the purposes of academic research, subject always to the full Conditions of use http://www.nature.com/authors/policies/license.html
Description
The project was supported by the UK Engineering and Physical Sciences Research Council, RS Macdonald Charitable Trust, Scottish Universities Life Sciences Alliance and St Andrews 600 anniversary BRAINS appeal. The PhD apprenticeship of C.C.L. was funded by the School of Biology.
Collections
  • University of St Andrews Research
URI
http://hdl.handle.net/10023/5521

Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

Advanced Search

Browse

All of RepositoryCommunities & CollectionsBy Issue DateNamesTitlesSubjectsClassificationTypeFunderThis CollectionBy Issue DateNamesTitlesSubjectsClassificationTypeFunder

My Account

Login

Open Access

To find out how you can benefit from open access to research, see our library web pages and Open Access blog. For open access help contact: openaccess@st-andrews.ac.uk.

Accessibility

Read our Accessibility statement.

How to submit research papers

The full text of research papers can be submitted to the repository via Pure, the University's research information system. For help see our guide: How to deposit in Pure.

Electronic thesis deposit

Help with deposit.

Repository help

For repository help contact: Digital-Repository@st-andrews.ac.uk.

Give Feedback

Cookie policy

This site may use cookies. Please see Terms and Conditions.

Usage statistics

COUNTER-compliant statistics on downloads from the repository are available from the IRUS-UK Service. Contact us for information.

© University of St Andrews Library

University of St Andrews is a charity registered in Scotland, No SC013532.

  • Facebook
  • Twitter