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dc.contributor.authorPérez-Moreno, J J
dc.contributor.authorBischoff, M
dc.contributor.authorMartín-Bermudo, M D
dc.contributor.authorEstrada, B
dc.date.accessioned2014-07-18T16:31:01Z
dc.date.available2014-07-18T16:31:01Z
dc.date.issued2014-07
dc.identifier.citationPérez-Moreno , J J , Bischoff , M , Martín-Bermudo , M D & Estrada , B 2014 , ' The conserved transmembrane proteoglycan Perdido/Kon-tiki is essential for myofibrillogenesis and sarcomeric structure in Drosophila ' , Journal of Cell Science , vol. 127 , pp. 3162-3173 . https://doi.org/10.1242/jcs.150425en
dc.identifier.issn0021-9533
dc.identifier.otherPURE: 123097998
dc.identifier.otherPURE UUID: e3b2afe1-bfe4-4068-991a-9fd92aea61e7
dc.identifier.otherPubMed: 24794494
dc.identifier.otherScopus: 84904291881
dc.identifier.otherORCID: /0000-0002-0737-6321/work/41120271
dc.identifier.urihttps://hdl.handle.net/10023/5040
dc.descriptionM.B. was funded by a Wellcome Trust Senior Investigator Award to P. Lawrence (WT096645MA).en
dc.description.abstractMuscle differentiation requires the assembly of high-order structures called myofibrils, composed of sarcomeres. Even though the molecular organization of sarcomeres is well known, the mechanisms underlying myofibrillogenesis are poorly understood. It has been proposed that integrin-dependent adhesion nucleates myofibril at the periphery of the muscle cell to sustain sarcomere assembly. Here, we report a role for the gene perdido (perd, also known as kon-tiki, a transmembrane chondroitin proteoglycan) in myofibrillogenesis. Expression of perd RNAi in muscles, prior to adult myogenesis, can induce misorientation and detachment of Drosophila adult abdominal muscles. In comparison to controls, perd-depleted muscles contain fewer myofibrils, localized at the cell periphery. These myofibrils are detached from each other and display a defective sarcomeric structure. Our results demonstrate that the extracellular matrix receptor Perd has a specific role in the assembly of myofibrils and in sarcomeric organization. We suggest that Perd acts downstream or in parallel to integrins to enable the connection of nascent myofibrils to the Z-bands. Our work identifies the Drosophila adult abdominal muscles as a model to investigate in vivo the mechanisms behind myofibrillogenesis.
dc.language.isoeng
dc.relation.ispartofJournal of Cell Scienceen
dc.rightsCopyright 2014. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.en
dc.subjectMyogenesisen
dc.subjectMuscleen
dc.subjectMyofibrilen
dc.subjectSarcomereen
dc.subjectIntegrinen
dc.subjectChondroitin sulfate proteoglycanen
dc.subjectPerdidoen
dc.subjectKon-tikien
dc.subjectCSPG4en
dc.subjectMCSPen
dc.subjectAN2en
dc.subjectNG2en
dc.subjectQR Microbiologyen
dc.subject.lccQRen
dc.titleThe conserved transmembrane proteoglycan Perdido/Kon-tiki is essential for myofibrillogenesis and sarcomeric structure in Drosophilaen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1242/jcs.150425
dc.description.statusPeer revieweden


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