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dc.contributor.authorSalmeron, Cristina
dc.contributor.authorGarcia de la Serrana Castillo, Daniel
dc.contributor.authorJimenez-Amilburu, Vanesa
dc.contributor.authorFontanillas, Ramon
dc.contributor.authorNavarro, Isabel
dc.contributor.authorJohnston, Ian A.
dc.contributor.authorGutierrez, Joaquim
dc.contributor.authorCapilla, Encarnacion
dc.date.accessioned2014-07-10T15:01:02Z
dc.date.available2014-07-10T15:01:02Z
dc.date.issued2013-09-25
dc.identifier.citationSalmeron , C , Garcia de la Serrana Castillo , D , Jimenez-Amilburu , V , Fontanillas , R , Navarro , I , Johnston , I A , Gutierrez , J & Capilla , E 2013 , ' Characterisation and expression of calpain family members in relation to nutritional status, diet composition and flesh texture in gilthead sea bream (Sparus aurata) ' PLoS One , vol. 8 , no. 9 , e75349 . https://doi.org/10.1371/journal.pone.0075349en
dc.identifier.issn1932-6203
dc.identifier.otherPURE: 131083077
dc.identifier.otherPURE UUID: f9ff2497-8a20-4589-acf7-5d766df19339
dc.identifier.otherWOS: 000325218700079
dc.identifier.otherScopus: 84884565009
dc.identifier.otherORCID: /0000-0002-7796-5754/work/47136011
dc.identifier.urihttp://hdl.handle.net/10023/5012
dc.descriptionEC is a Ramón y Cajal researcher fellow from the “Ministerio de Ciencia e Innovaci” (MICINN). This work was supported by funds from the MICINN (AGL2009-12427 and AGL2010-17324), the Catalonian Government (2009SGR-00402) and the “Xarxa de Referència d’R+D+I en Aqüicultura” and the European Union through the project LIFECYCLE (FP7-222719).en
dc.description.abstractCalpains are non-lysosomal calcium-activated neutral proteases involved in a wide range of cellular processes including muscle proteolysis linked to post-mortem flesh softening. The aims of this study were (a) to characterise several members of the calpain system in gilthead sea bream and (b) to examine their expression in relation to nutritional status and muscle tenderisation. We identified the complete open reading frame of gilthead sea bream calpains1-3, sacapn1, sacapn2, sacapn3, and two paralogs of the calpain small subunit1, sacapns1a and sacapns1b. Proteins showed 63-90% sequence identity compared with sequences from mammals and other teleost fishes, and the characteristic domain structure of vertebrate calpains. Transcripts of sacapn1, sacapn2, sacapns1a and sacapns1b had a wide tissue distribution, whereas sacapn3 was almost exclusively detected in skeletal muscle. Next, we assessed transcript expression in skeletal muscle following alteration of nutritional status by (a) fasting and re-feeding or (b) feeding four experimental diets with different carbohydrate-to-protein ratios. Fasting significantly reduced plasma glucose and increased free fatty acids and triglycerides, together with a significant increase in sacapns1b expression. Following 7 days of re-feeding, plasma parameters returned to fed values and sacapn1, sacapn2, sacapns1a and sacapns1b expression was significantly reduced. Furthermore, an increase in dietary carbohydrate content (11 to 39%) diminished growth but increased muscle texture, which showed a significant correlation with decreased sacapn1 and sacapns1a expression, whilst the other calpains remained unaffected. This study has demonstrated that calpain expression is modulated by nutritional status and diet composition in gilthead sea bream, and that the expression of several calpain members is correlated with muscle texture, indicating their potential use as molecular markers for flesh quality in aquaculture production.en
dc.format.extent15en
dc.language.isoeng
dc.relation.ispartofPLoS Oneen
dc.rightsCopyright: © 2013 Salmerón et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. http://creativecommons.org/licenses/by/4.0/en
dc.subjectTrout oncorhynchus-mykissen
dc.subjectCalcium-dependent proteaseen
dc.subjectBass dicentrarchus-labraxen
dc.subjectMuscle-specific calpainen
dc.subjectSkeletal-muscleen
dc.subjectAtlantic salmonen
dc.subjectGrowth dynamicsen
dc.subjectMyofibril fragmentationen
dc.subjectLongissimus muscleen
dc.subjectMessenger-RNAen
dc.subjectQH301 Biologyen
dc.subject.lccQH301en
dc.titleCharacterisation and expression of calpain family members in relation to nutritional status, diet composition and flesh texture in gilthead sea bream (Sparus aurata)en
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Marine Alliance for Science & Technology Scotlanden
dc.contributor.institutionUniversity of St Andrews. Scottish Oceans Instituteen
dc.contributor.institutionUniversity of St Andrews. Centre for Research into Ecological & Environmental Modellingen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0075349
dc.description.statusPeer revieweden


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