Show simple item record

Files in this item


Item metadata

dc.contributor.authorBossé, Janine T
dc.contributor.authorLi, Yanwen
dc.contributor.authorAngen, Oystein
dc.contributor.authorWeinert, Lucy A
dc.contributor.authorChaudhuri, Roy R
dc.contributor.authorHolden, Matt T
dc.contributor.authorWilliamson, Susanna M
dc.contributor.authorMaskell, Duncan J
dc.contributor.authorTucker, Alexander W
dc.contributor.authorWren, Brendan W
dc.contributor.authorRycroft, Andrew N
dc.contributor.authorLangford, Paul R
dc.contributor.authorBRaDP1T Consortium
dc.contributor.authorHolden, Matthew
dc.identifier.citationBossé , J T , Li , Y , Angen , O , Weinert , L A , Chaudhuri , R R , Holden , M T , Williamson , S M , Maskell , D J , Tucker , A W , Wren , B W , Rycroft , A N , Langford , P R , BRaDP1T Consortium & Holden , M 2014 , ' Multiplex PCR Assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12 ' , Journal of Clinical Microbiology , pp. 2380-2385 .
dc.identifier.otherPURE: 115032868
dc.identifier.otherPURE UUID: aa9f9863-79c4-4589-9854-47de330cd3c4
dc.identifier.otherPubMed: 24759717
dc.identifier.otherScopus: 84903760703
dc.identifier.otherORCID: /0000-0002-4958-2166/work/60196496
dc.descriptionThis work was supported by a Longer and Larger (LoLa) grant from the Biotechnology and Biological Sciences Research Council (grant numbers BB/G020744/1, BB/G019177/1, BB/G019274/1, and BB/G003203/1) and the United Kingdom Department for Environment, Food and Rural Af-fairs and Zoetis awarded to the Bacterial Respiratory Diseases of Pigs-1 Technology(BRaDP1T)consortium.en
dc.description.abstractAn improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovar 3, 6 and 8 Actinobacillus pleuropneumoniae isolates is described. The new primers eliminate an aberrant serovar 3 indicative amplicon found in some serovar 6 clinical isolates. Furthermore, we have developed a new multiplex PCR for detection of serovars 1-3, 5-8, 10, and 12 along with apxIV, thus extending the utility of this diagnostic PCR to cover a broader range of isolates.
dc.relation.ispartofJournal of Clinical Microbiologyen
dc.rightsCopyright © 2014 Bossé et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license.
dc.subjectQR Microbiologyen
dc.titleMultiplex PCR Assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12en
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Medicineen
dc.contributor.institutionUniversity of St Andrews.Infection Groupen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.description.statusPeer revieweden

This item appears in the following Collection(s)

Show simple item record