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Multiplex PCR Assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12

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dc.contributor.authorBossé, Janine T
dc.contributor.authorLi, Yanwen
dc.contributor.authorAngen, Oystein
dc.contributor.authorWeinert, Lucy A
dc.contributor.authorChaudhuri, Roy R
dc.contributor.authorHolden, Matt T
dc.contributor.authorWilliamson, Susanna M
dc.contributor.authorMaskell, Duncan J
dc.contributor.authorTucker, Alexander W
dc.contributor.authorWren, Brendan W
dc.contributor.authorRycroft, Andrew N
dc.contributor.authorLangford, Paul R
dc.contributor.authorBRaDP1T Consortium
dc.contributor.authorHolden, Matthew
dc.date.accessioned2014-07-08T11:31:03Z
dc.date.available2014-07-08T11:31:03Z
dc.date.issued2014-07
dc.identifier.citationBossé , J T , Li , Y , Angen , O , Weinert , L A , Chaudhuri , R R , Holden , M T , Williamson , S M , Maskell , D J , Tucker , A W , Wren , B W , Rycroft , A N , Langford , P R , BRaDP1T Consortium & Holden , M 2014 , ' Multiplex PCR Assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12 ' , Journal of Clinical Microbiology , pp. 2380-2385 . https://doi.org/10.1128/JCM.00685-14en
dc.identifier.issn0095-1137
dc.identifier.otherPURE: 115032868
dc.identifier.otherPURE UUID: aa9f9863-79c4-4589-9854-47de330cd3c4
dc.identifier.otherPubMed: 24759717
dc.identifier.otherScopus: 84903760703
dc.identifier.otherORCID: /0000-0002-4958-2166/work/60196496
dc.identifier.urihttps://hdl.handle.net/10023/4971
dc.descriptionThis work was supported by a Longer and Larger (LoLa) grant from the Biotechnology and Biological Sciences Research Council (grant numbers BB/G020744/1, BB/G019177/1, BB/G019274/1, and BB/G003203/1) and the United Kingdom Department for Environment, Food and Rural Af-fairs and Zoetis awarded to the Bacterial Respiratory Diseases of Pigs-1 Technology(BRaDP1T)consortium.en
dc.description.abstractAn improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovar 3, 6 and 8 Actinobacillus pleuropneumoniae isolates is described. The new primers eliminate an aberrant serovar 3 indicative amplicon found in some serovar 6 clinical isolates. Furthermore, we have developed a new multiplex PCR for detection of serovars 1-3, 5-8, 10, and 12 along with apxIV, thus extending the utility of this diagnostic PCR to cover a broader range of isolates.
dc.format.extent6
dc.language.isoeng
dc.relation.ispartofJournal of Clinical Microbiologyen
dc.rightsCopyright © 2014 Bossé et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license. https://creativecommons.org/licenses/by/3.0/en
dc.subjectQR Microbiologyen
dc.subject.lccQRen
dc.titleMultiplex PCR Assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12en
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. Infection Groupen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1128/JCM.00685-14
dc.description.statusPeer revieweden


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