Multiplex PCR Assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12
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An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovar 3, 6 and 8 Actinobacillus pleuropneumoniae isolates is described. The new primers eliminate an aberrant serovar 3 indicative amplicon found in some serovar 6 clinical isolates. Furthermore, we have developed a new multiplex PCR for detection of serovars 1-3, 5-8, 10, and 12 along with apxIV, thus extending the utility of this diagnostic PCR to cover a broader range of isolates.
Bossé , J T , Li , Y , Angen , O , Weinert , L A , Chaudhuri , R R , Holden , M T , Williamson , S M , Maskell , D J , Tucker , A W , Wren , B W , Rycroft , A N , Langford , P R , BRaDP1T Consortium & Holden , M 2014 , ' Multiplex PCR Assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12 ' Journal of Clinical Microbiology , pp. 2380-2385 . DOI: 10.1128/JCM.00685-14
Journal of Clinical Microbiology
Copyright © 2014 Bossé et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license. https://creativecommons.org/licenses/by/3.0/
DescriptionThis work was supported by a Longer and Larger (LoLa) grant from the Biotechnology and Biological Sciences Research Council (grant numbers BB/G020744/1, BB/G019177/1, BB/G019274/1, and BB/G003203/1) and the United Kingdom Department for Environment, Food and Rural Af-fairs and Zoetis awarded to the Bacterial Respiratory Diseases of Pigs-1 Technology(BRaDP1T)consortium.
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