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dc.contributor.authorZhang, Jing
dc.contributor.authorWhite, Malcolm F.
dc.date.accessioned2014-06-30T23:01:34Z
dc.date.available2014-06-30T23:01:34Z
dc.date.issued2013-12
dc.identifier91039041
dc.identifier1a5673ea-f41a-4fdb-9abb-78a299731c74
dc.identifier000328282700010
dc.identifier84887868612
dc.identifier.citationZhang , J & White , M F 2013 , ' Hot and crispy : CRISPR-Cas systems in the hyperthermophile Sulfolobus solfataricus ' , Biochemical Society Transactions , vol. 41 , no. 6 , pp. 1422-1426 . https://doi.org/10.1042/BST20130031en
dc.identifier.issn0300-5127
dc.identifier.otherORCID: /0000-0003-1543-9342/work/47136103
dc.identifier.urihttps://hdl.handle.net/10023/4930
dc.description.abstractThe CRISPR (clustered regularly interspaced short palindromic repeats) and Cas (CRISPR-associated) genes are widely spread in bacteria and archaea, representing an intracellular defence system against invading viruses and plasmids. In the system, fragments from foreign DNA are captured and integrated into the host genome at the CRISPR locus. The locus is transcribed and the resulting RNAs are processed by Cas6 into small crRNAs (CRISPR RNAs) that guide a variety of effector complexes to degrade the invading genetic elements. Many bacteria and archaea have one major type of effector complex. However, Sulfolobus solfataricus strain P2 has six CRISPR loci with two families of repeats, four cas6 genes and three different types of effector complex. These features make S. solfataricus an important model for studying CRISPR-Cas systems. In the present article, we review our current understanding of crRNA biogenesis and its effector complexes, subtype I-A and subtype III-B, in S. solfataricus. We also discuss the differences in terms of mechanisms between the subtype III-B systems in S. solfataricus and Pyrococcus furiosus.
dc.format.extent5
dc.format.extent403140
dc.language.isoeng
dc.relation.ispartofBiochemical Society Transactionsen
dc.subjectCas6en
dc.subjectClustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated (Cas) systemen
dc.subjectCRISPR-associated complex for antiviral defence (Cascade)en
dc.subjectCRISPR-module repeat-associated mysterious protein (CMR)en
dc.subjectCRISPR RNA (crRNA)en
dc.subjectSulfolobus solfataricusen
dc.titleHot and crispy : CRISPR-Cas systems in the hyperthermophile Sulfolobus solfataricusen
dc.typeJournal articleen
dc.contributor.sponsorBBSRCen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1042/BST20130031
dc.description.statusPeer revieweden
dc.date.embargoedUntil2014-07-01
dc.identifier.urlhttp://www.biochemsoctrans.org/bst/041/bst0411422.htmen
dc.identifier.grantnumberBB/K000314/1en


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