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Structure of PatF from Prochloron didemni
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dc.contributor.author | Bent, Andrew F. | |
dc.contributor.author | Koehnke, Jesko | |
dc.contributor.author | Houssen, Wael E. | |
dc.contributor.author | Smith, Margaret C. M. | |
dc.contributor.author | Jaspars, Marcel | |
dc.contributor.author | Naismith, James H. | |
dc.date.accessioned | 2014-05-28T15:01:02Z | |
dc.date.available | 2014-05-28T15:01:02Z | |
dc.date.issued | 2013-05-23 | |
dc.identifier | 122502486 | |
dc.identifier | d0b43721-97c0-462b-98ce-e57e9beda033 | |
dc.identifier | 000319736000006 | |
dc.identifier | 84878570912 | |
dc.identifier.citation | Bent , A F , Koehnke , J , Houssen , W E , Smith , M C M , Jaspars , M & Naismith , J H 2013 , ' Structure of PatF from Prochloron didemni ' , Acta Crystallographica. Section F, Structural biology and crystallization communications , vol. F69 , pp. 618-623 . https://doi.org/10.1107/S1744309113012931 | en |
dc.identifier.issn | 1744-3091 | |
dc.identifier.uri | https://hdl.handle.net/10023/4826 | |
dc.description | This work was funded by the Leverhulme Trust (RPG-2012-504) and BBSRC (BB/K015508/1); the University of St Andrews infrastructure is supported by a Wellcome Trust Capital Award (086036). | en |
dc.description.abstract | Patellamides are macrocyclic peptides with potent biological effects and are a subset of the cyanobactins. Cyanobactins are natural products that are produced by a series of enzymatic transformations and a common modification is the addition of a prenyl group. Puzzlingly, the pathway for patellamides in Prochloron didemni contains a gene, patF, with homology to prenylases, but patellamides are not themselves prenylated. The structure of the protein PatF was cloned, expressed, purified and determined. Prenylase activity could not be demonstrated for the protein, and examination of the structure revealed changes in side-chain identity at the active site. It is suggested that these changes have inactivated the protein. Attempts to mutate these residues led to unfolded protein. | |
dc.format.extent | 6 | |
dc.format.extent | 1236637 | |
dc.language.iso | eng | |
dc.relation.ispartof | Acta Crystallographica. Section F, Structural biology and crystallization communications | en |
dc.subject | Macromolecular crystallography | en |
dc.subject | Lissoclinum-patella | en |
dc.subject | Refinement | en |
dc.subject | Quality | en |
dc.subject | System | en |
dc.subject | QD Chemistry | en |
dc.subject.lcc | QD | en |
dc.title | Structure of PatF from Prochloron didemni | en |
dc.type | Journal article | en |
dc.contributor.sponsor | BBSRC | en |
dc.contributor.institution | University of St Andrews. School of Chemistry | en |
dc.contributor.institution | University of St Andrews. EaSTCHEM | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | 10.1107/S1744309113012931 | |
dc.description.status | Peer reviewed | en |
dc.identifier.grantnumber | BB/K015508/1 | en |
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