Structure of PatF from Prochloron didemni
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Patellamides are macrocyclic peptides with potent biological effects and are a subset of the cyanobactins. Cyanobactins are natural products that are produced by a series of enzymatic transformations and a common modification is the addition of a prenyl group. Puzzlingly, the pathway for patellamides in Prochloron didemni contains a gene, patF, with homology to prenylases, but patellamides are not themselves prenylated. The structure of the protein PatF was cloned, expressed, purified and determined. Prenylase activity could not be demonstrated for the protein, and examination of the structure revealed changes in side-chain identity at the active site. It is suggested that these changes have inactivated the protein. Attempts to mutate these residues led to unfolded protein.
Bent , A F , Koehnke , J , Houssen , W E , Smith , M C M , Jaspars , M & Naismith , J H 2013 , ' Structure of PatF from Prochloron didemni ' Acta Crystallographica. Section F, Structural biology and crystallization communications , vol F69 , pp. 618-623 . DOI: 10.1107/S1744309113012931
Acta Crystallographica. Section F, Structural biology and crystallization communications
© 2013 The Authors. This is an open access article under the CC-BY license
This work was funded by the Leverhulme Trust (RPG-2012-504) and BBSRC (BB/K015508/1); the University of St Andrews infrastructure is supported by a Wellcome Trust Capital Award (086036).
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