A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods
MetadataShow full item record
Background: Cell migration is a complex phenomenon that requires the coordination of numerous cellular processes. Investigation of cell migration and its underlying biology is of interest to basic scientists and those in search of therapeutics. Current migration assays for screening small molecules, siRNAs, or other perturbations are difficult to perform in parallel at the scale required to screen large libraries. Results: We have adapted the commonly used scratch wound healing assay of tissue-culture cell monolayers to a 384 well plate format. By mechanically scratching the cell substrate with a pin array, we are able to create characteristically sized wounds in all wells of a 384 well plate. Imaging of the healing wounds with an automated fluorescence microscope allows us to distinguish perturbations that affect cell migration, morphology, and division. Readout requires similar to1 hr per plate but is high in information content i.e. high content. We compare readouts using different imaging technologies, automated microscopy, scanners and a fluorescence macroscope, and evaluate the trade-off between information content and data acquisition rate. Conclusions: The adaptation of a wound healing assay to a 384 well format facilitates the study of aspects of cell migration, tissue reorganization, cell division, and other processes that underlie wound healing. This assay allows greater than 10,000 perturbations to be screened per day with a quantitative, high-content readout, and can also be used to characterize small numbers of perturbations in detail.
Yarrow , J C , Perlman , Z E , Westwood , N J & Mitchison , T J 2004 , ' A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods ' BMC Biotechnology , vol 4 , 21 . DOI: 10.1186/1472-6750-4-21
© 2004 Yarrow et al; licensee BioMed Central Ltd. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
DescriptionJCY and ZEP were supported by Howard Hughes Medical Institute pre-doctoral fellowships. Funding provided to TJM through NIH GM048027-12.
Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.