Show simple item record

Files in this item

Thumbnail

Item metadata

dc.contributor.authorArita, Yoshihiko
dc.contributor.authorTorres-Mapa, Maria Leilani
dc.contributor.authorLee, Woei Ming
dc.contributor.authorCizmar, Tomas
dc.contributor.authorCampbell, Paul A
dc.contributor.authorGunn-Moore, Frank J
dc.contributor.authorDholakia, Kishan
dc.date.accessioned2014-01-10T10:31:04Z
dc.date.available2014-01-10T10:31:04Z
dc.date.issued2011-03
dc.identifier5105111
dc.identifier5cfd54ff-daea-43db-baf4-fcac0590b5e3
dc.identifier79952407617
dc.identifier.citationArita , Y , Torres-Mapa , M L , Lee , W M , Cizmar , T , Campbell , P A , Gunn-Moore , F J & Dholakia , K 2011 , ' Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles ' , Applied Physics Letters , vol. 98 , no. 9 , 093702 . https://doi.org/10.1063/1.3554415en
dc.identifier.issn0003-6951
dc.identifier.otherORCID: /0000-0003-3422-3387/work/34730454
dc.identifier.urihttps://hdl.handle.net/10023/4340
dc.description.abstractWe demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique.
dc.format.extent3
dc.format.extent708964
dc.language.isoeng
dc.relation.ispartofApplied Physics Lettersen
dc.subjectNanoparticlesen
dc.subjectCell membranesen
dc.subjectCavitationen
dc.subjectCell membrane transporten
dc.subjectMonolayersen
dc.titleSpatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticlesen
dc.typeJournal articleen
dc.contributor.sponsorEPSRCen
dc.contributor.institutionUniversity of St Andrews. School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. Institute of Behavioural and Neural Sciencesen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1063/1.3554415
dc.description.statusPeer revieweden
dc.identifier.grantnumberEP/G029733/1en


This item appears in the following Collection(s)

Show simple item record