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Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles
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dc.contributor.author | Arita, Yoshihiko | |
dc.contributor.author | Torres-Mapa, Maria Leilani | |
dc.contributor.author | Lee, Woei Ming | |
dc.contributor.author | Cizmar, Tomas | |
dc.contributor.author | Campbell, Paul A | |
dc.contributor.author | Gunn-Moore, Frank J | |
dc.contributor.author | Dholakia, Kishan | |
dc.date.accessioned | 2014-01-10T10:31:04Z | |
dc.date.available | 2014-01-10T10:31:04Z | |
dc.date.issued | 2011-03 | |
dc.identifier | 5105111 | |
dc.identifier | 5cfd54ff-daea-43db-baf4-fcac0590b5e3 | |
dc.identifier | 79952407617 | |
dc.identifier.citation | Arita , Y , Torres-Mapa , M L , Lee , W M , Cizmar , T , Campbell , P A , Gunn-Moore , F J & Dholakia , K 2011 , ' Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles ' , Applied Physics Letters , vol. 98 , no. 9 , 093702 . https://doi.org/10.1063/1.3554415 | en |
dc.identifier.issn | 0003-6951 | |
dc.identifier.other | ORCID: /0000-0003-3422-3387/work/34730454 | |
dc.identifier.uri | https://hdl.handle.net/10023/4340 | |
dc.description.abstract | We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique. | |
dc.format.extent | 3 | |
dc.format.extent | 708964 | |
dc.language.iso | eng | |
dc.relation.ispartof | Applied Physics Letters | en |
dc.subject | Nanoparticles | en |
dc.subject | Cell membranes | en |
dc.subject | Cavitation | en |
dc.subject | Cell membrane transport | en |
dc.subject | Monolayers | en |
dc.title | Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles | en |
dc.type | Journal article | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.institution | University of St Andrews. School of Physics and Astronomy | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.contributor.institution | University of St Andrews. School of Medicine | en |
dc.contributor.institution | University of St Andrews. Institute of Behavioural and Neural Sciences | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | 10.1063/1.3554415 | |
dc.description.status | Peer reviewed | en |
dc.identifier.grantnumber | EP/G029733/1 | en |
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