Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles
Abstract
We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique.
Citation
Arita , Y , Torres-Mapa , M L , Lee , W M , Cizmar , T , Campbell , P A , Gunn-Moore , F J & Dholakia , K 2011 , ' Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles ' , Applied Physics Letters , vol. 98 , no. 9 , 093702 . https://doi.org/10.1063/1.3554415
Publication
Applied Physics Letters
Status
Peer reviewed
ISSN
0003-6951Type
Journal article
Collections
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