Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles
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We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique.
Arita , Y , Torres-Mapa , M L , Lee , W M , Cizmar , T , Campbell , P A , Gunn-Moore , F J & Dholakia , K 2011 , ' Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles ' Applied Physics Letters , vol 98 , no. 9 , 093702 . DOI: 10.1063/1.3554415
Applied Physics Letters
Copyright 2011, American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in Applied Physics Letters, Vol 98, Issue 9, and may be found at: http://scitation.aip.org/content/aip/journal/apl/98/9/10.1063/1.3554415
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