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Wavefront corrected light sheet microscopy in turbid media
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dc.contributor.author | Dalgarno, Heather Isla Campbell | |
dc.contributor.author | Cizmar, Tomas | |
dc.contributor.author | Vettenburg, Tom | |
dc.contributor.author | Nylk, Jonathan | |
dc.contributor.author | Gunn-Moore, Frank J | |
dc.contributor.author | Dholakia, Kishan | |
dc.date.accessioned | 2013-08-06T12:01:02Z | |
dc.date.available | 2013-08-06T12:01:02Z | |
dc.date.issued | 2012-05 | |
dc.identifier | 19418573 | |
dc.identifier | b854883f-2c88-4859-8983-0e0528d6e9df | |
dc.identifier | 84862099416 | |
dc.identifier.citation | Dalgarno , H I C , Cizmar , T , Vettenburg , T , Nylk , J , Gunn-Moore , F J & Dholakia , K 2012 , ' Wavefront corrected light sheet microscopy in turbid media ' , Applied Physics Letters , vol. 100 , no. 19 , 191108 . https://doi.org/10.1063/1.4710527 | en |
dc.identifier.issn | 0003-6951 | |
dc.identifier.other | ORCID: /0000-0003-3422-3387/work/34730445 | |
dc.identifier.other | ORCID: /0000-0002-2977-4929/work/41304437 | |
dc.identifier.uri | https://hdl.handle.net/10023/3932 | |
dc.description | This project was supported by the UK Engineering and Physical Sciences Research Council. | en |
dc.description.abstract | Light sheet microscopy is a powerful method for three-dimensional imaging of large biological specimens. However, its imaging ability is greatly diminished by sample scattering and aberrations. Optical clearing, Bessel light modes, and background rejection have been employed in attempts to circumvent these deleterious effects. We present an in situ wavefront correction that offers a major advance by creating an “optimal” light sheet within a turbid sample. Crucially, we show that no tissue clearing or specialized sample preparation is required, and clear improvements in image quality and depth resolution are demonstrated both in Gaussian and Bessel beam-based light sheet modalities. | |
dc.format.extent | 1061891 | |
dc.language.iso | eng | |
dc.relation.ispartof | Applied Physics Letters | en |
dc.subject | Light sheet microscopy | en |
dc.subject | Optical clearing | en |
dc.subject | Bessel light modes | en |
dc.subject | Background rejection | en |
dc.subject | Wavefront correction | en |
dc.subject | Gaussian beam | en |
dc.subject | Bessel beam | en |
dc.subject | Q Science | en |
dc.subject.lcc | Q | en |
dc.title | Wavefront corrected light sheet microscopy in turbid media | en |
dc.type | Journal article | en |
dc.contributor.institution | University of St Andrews. School of Physics and Astronomy | en |
dc.contributor.institution | University of St Andrews. School of Medicine | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.contributor.institution | University of St Andrews. Institute of Behavioural and Neural Sciences | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | 10.1063/1.4710527 | |
dc.description.status | Peer reviewed | en |
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