Wavefront corrected light sheet microscopy in turbid media
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Light sheet microscopy is a powerful method for three-dimensional imaging of large biological specimens. However, its imaging ability is greatly diminished by sample scattering and aberrations. Optical clearing, Bessel light modes, and background rejection have been employed in attempts to circumvent these deleterious effects. We present an in situ wavefront correction that offers a major advance by creating an “optimal” light sheet within a turbid sample. Crucially, we show that no tissue clearing or specialized sample preparation is required, and clear improvements in image quality and depth resolution are demonstrated both in Gaussian and Bessel beam-based light sheet modalities.
Dalgarno , H I C , Cizmar , T , Vettenburg , T , Nylk , J , Gunn-Moore , F J & Dholakia , K 2012 , ' Wavefront corrected light sheet microscopy in turbid media ' Applied Physics Letters , vol. 100 , no. 19 , 191108 . DOI: 10.1063/1.4710527
Applied Physics Letters
© 2012 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in Applied Physics Letters, Vol 100, Issue 19, and may be found online at: http://scitation.aip.org/content/aip/journal/apl/100/19/10.1063/1.4710527
DescriptionThis project was supported by the UK Engineering and Physical Sciences Research Council.
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