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dc.contributor.authorBailey, Nathan William
dc.contributor.authorVeltsos, Paris
dc.contributor.authorTan, Yew-Foon
dc.contributor.authorMillar, A. Harvey
dc.contributor.authorRitchie, Michael Gordon
dc.contributor.authorSimmons, Leigh W.
dc.identifier.citationBailey , N W , Veltsos , P , Tan , Y-F , Millar , A H , Ritchie , M G & Simmons , L W 2013 , ' Tissue-specific transcriptomics in the field cricket Teleogryllus oceanicus ' , G3: Genes, Genomes, Genetics , vol. 3 , no. 2 , pp. 225-230 .
dc.identifier.otherPURE: 47729774
dc.identifier.otherPURE UUID: f3a4f1e9-0bfa-40af-b3e4-a2557433fd8a
dc.identifier.otherScopus: 84883180924
dc.identifier.otherORCID: /0000-0001-7913-8675/work/46761111
dc.identifier.otherORCID: /0000-0003-3531-7756/work/60888431
dc.descriptionThis work was supported by a Natural Environment Research Council Junior Postdoctoral Fellowship (NE/G014906/1) and Pacific Rim Foundation funding (08-T-PRRP-05-0029) to N.W.B.en
dc.description.abstractField crickets (family Gryllidae) frequently are used in studies of behavioral genetics, sexual selection, and sexual conflict, but there have been no studies of transcriptomic differences among different tissue types. We evaluated transcriptome variation among testis, accessory gland, and the remaining whole-body preparations from males of the field cricket, Teleogryllus oceanicus. Non-normalized cDNA libraries from each tissue were sequenced on the Roche 454 platform, and a master assembly was constructed using testis, accessory gland, and whole-body preparations. A total of 940,200 reads were assembled into 41,962 contigs, to which 36,856 singletons (reads not assembled into a contig) were added to provide a total of 78,818 sequences used in annotation analysis. A total of 59,072 sequences (75%) were unique to one of the three tissues. Testis tissue had the greatest proportion of tissue-specific sequences (62.6%), followed by general body (56.43%) and accessory gland tissue (44.16%). We tested the hypothesis that tissues expressing gene products expected to evolve rapidly as a result of sexual selection—testis and accessory gland—would yield a smaller proportion of BLASTx matches to homologous genes in the model organism Drosophila melanogaster compared with whole-body tissue. Uniquely expressed sequences in both testis and accessory gland showed a significantly lower rate of matching to annotated D. melanogaster genes compared with those from general body tissue. These results correspond with empirical evidence that genes expressed in testis and accessory gland tissue are rapidly evolving targets of selection.
dc.relation.ispartofG3: Genes, Genomes, Geneticsen
dc.rightsCopyright © 2013 Bailey et al. This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.subjectField cricketen
dc.subjectTeleogryllus oceanicusen
dc.subjectTissue-specific transcriptomeen
dc.subjectQH426 Geneticsen
dc.titleTissue-specific transcriptomics in the field cricket Teleogryllus oceanicusen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Centre for Biological Diversityen
dc.contributor.institutionUniversity of St Andrews. Institute of Behavioural and Neural Sciencesen
dc.description.statusPeer revieweden

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