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Tissue-specific transcriptomics in the field cricket Teleogryllus oceanicus

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Date
01/02/2013
Author
Bailey, Nathan William
Veltsos, Paris
Tan, Yew-Foon
Millar, A. Harvey
Ritchie, Michael Gordon
Simmons, Leigh W.
Funder
NERC
Grant ID
NE/G014906/1
Keywords
Field cricket
Gryllid
Teleogryllus oceanicus
Tissue-specific transcriptome
QH426 Genetics
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Abstract
Field crickets (family Gryllidae) frequently are used in studies of behavioral genetics, sexual selection, and sexual conflict, but there have been no studies of transcriptomic differences among different tissue types. We evaluated transcriptome variation among testis, accessory gland, and the remaining whole-body preparations from males of the field cricket, Teleogryllus oceanicus. Non-normalized cDNA libraries from each tissue were sequenced on the Roche 454 platform, and a master assembly was constructed using testis, accessory gland, and whole-body preparations. A total of 940,200 reads were assembled into 41,962 contigs, to which 36,856 singletons (reads not assembled into a contig) were added to provide a total of 78,818 sequences used in annotation analysis. A total of 59,072 sequences (75%) were unique to one of the three tissues. Testis tissue had the greatest proportion of tissue-specific sequences (62.6%), followed by general body (56.43%) and accessory gland tissue (44.16%). We tested the hypothesis that tissues expressing gene products expected to evolve rapidly as a result of sexual selection—testis and accessory gland—would yield a smaller proportion of BLASTx matches to homologous genes in the model organism Drosophila melanogaster compared with whole-body tissue. Uniquely expressed sequences in both testis and accessory gland showed a significantly lower rate of matching to annotated D. melanogaster genes compared with those from general body tissue. These results correspond with empirical evidence that genes expressed in testis and accessory gland tissue are rapidly evolving targets of selection.
Citation
Bailey , N W , Veltsos , P , Tan , Y-F , Millar , A H , Ritchie , M G & Simmons , L W 2013 , ' Tissue-specific transcriptomics in the field cricket Teleogryllus oceanicus ' , G3: Genes, Genomes, Genetics , vol. 3 , no. 2 , pp. 225-230 . https://doi.org/10.1534/g3.112.004341
Publication
G3: Genes, Genomes, Genetics
Status
Peer reviewed
DOI
https://doi.org/10.1534/g3.112.004341
ISSN
2160-1836
Type
Journal article
Rights
Copyright © 2013 Bailey et al. This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Description
This work was supported by a Natural Environment Research Council Junior Postdoctoral Fellowship (NE/G014906/1) and Pacific Rim Foundation funding (08-T-PRRP-05-0029) to N.W.B.
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  • University of St Andrews Research
URI
http://hdl.handle.net/10023/3391

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