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dc.contributor.authorStramer, Brian
dc.contributor.authorMoreira, Severina
dc.contributor.authorMillard, Tom
dc.contributor.authorEvans, Iwan
dc.contributor.authorHuang, Chieh-Yin
dc.contributor.authorSabet, Ola
dc.contributor.authorMilner, Martin John
dc.contributor.authorDunn, Graham
dc.contributor.authorMartin, Paul
dc.contributor.authorWood, Will
dc.date.accessioned2012-07-18T11:01:05Z
dc.date.available2012-07-18T11:01:05Z
dc.date.issued2010-05-17
dc.identifier3437605
dc.identifier64f8208f-b66e-4711-91bb-28397bd7d088
dc.identifier77952335884
dc.identifier.citationStramer , B , Moreira , S , Millard , T , Evans , I , Huang , C-Y , Sabet , O , Milner , M J , Dunn , G , Martin , P & Wood , W 2010 , ' Clasp-mediated microtubule bundling regulates persistent motility and contact repulsion in Drosophila macrophages in vivo ' , Journal of Cell Biology , vol. 189 , no. 4 , pp. 681-689 . https://doi.org/10.1083/jcb.200912134en
dc.identifier.issn0021-9525
dc.identifier.urihttps://hdl.handle.net/10023/2999
dc.descriptionP. Martin and W. Wood contributed equally to this paperen
dc.description.abstractDrosophila melanogaster macrophages are highly migratory cells that lend themselves beautifully to high resolution in vivo imaging experiments. By expressing fluorescent probes to reveal actin and microtubules, we can observe the dynamic interplay of these two cytoskeletal networks as macrophages migrate and interact with one another within a living organism. We show that before an episode of persistent motility, whether responding to developmental guidance or wound cues, macrophages assemble a polarized array of microtubules that bundle into a compass-like arm that appears to anticipate the direction of migration. Whenever cells collide with one another, their microtubule arms transiently align just before cell–cell repulsion, and we show that forcing depolymerization of microtubules by expression of Spastin leads to their defective polarity and failure to contact inhibit from one another. The same is true in orbit/clasp mutants, indicating a pivotal role for this microtubule-binding protein in the assembly and/or functioning of the microtubule arm during polarized migration and contact repulsion.
dc.format.extent2904229
dc.language.isoeng
dc.relation.ispartofJournal of Cell Biologyen
dc.subjectQH301 Biologyen
dc.subject.lccQH301en
dc.titleClasp-mediated microtubule bundling regulates persistent motility and contact repulsion in Drosophila macrophages in vivoen
dc.typeJournal articleen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.identifier.doihttps://doi.org/10.1083/jcb.200912134
dc.description.statusPeer revieweden
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=77952335884&partnerID=8YFLogxKen
dc.identifier.urlhttp://jcb.rupress.org/content/189/4/681.abstracten


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