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Clasp-mediated microtubule bundling regulates persistent motility and contact repulsion in Drosophila macrophages in vivo

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Stramer2010JCBClaspMediated.pdf (2.769Mb)
Date
17/05/2010
Author
Stramer, Brian
Moreira, Severina
Millard, Tom
Evans, Iwan
Huang, Chieh-Yin
Sabet, Ola
Milner, Martin John
Dunn, Graham
Martin, Paul
Wood, Will
Keywords
QH301 Biology
Metadata
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Abstract
Drosophila melanogaster macrophages are highly migratory cells that lend themselves beautifully to high resolution in vivo imaging experiments. By expressing fluorescent probes to reveal actin and microtubules, we can observe the dynamic interplay of these two cytoskeletal networks as macrophages migrate and interact with one another within a living organism. We show that before an episode of persistent motility, whether responding to developmental guidance or wound cues, macrophages assemble a polarized array of microtubules that bundle into a compass-like arm that appears to anticipate the direction of migration. Whenever cells collide with one another, their microtubule arms transiently align just before cell–cell repulsion, and we show that forcing depolymerization of microtubules by expression of Spastin leads to their defective polarity and failure to contact inhibit from one another. The same is true in orbit/clasp mutants, indicating a pivotal role for this microtubule-binding protein in the assembly and/or functioning of the microtubule arm during polarized migration and contact repulsion.
Citation
Stramer , B , Moreira , S , Millard , T , Evans , I , Huang , C-Y , Sabet , O , Milner , M J , Dunn , G , Martin , P & Wood , W 2010 , ' Clasp-mediated microtubule bundling regulates persistent motility and contact repulsion in Drosophila macrophages in vivo ' , Journal of Cell Biology , vol. 189 , no. 4 , pp. 681-689 . https://doi.org/10.1083/jcb.200912134
Publication
Journal of Cell Biology
Status
Peer reviewed
DOI
https://doi.org/10.1083/jcb.200912134
ISSN
0021-9525
Type
Journal article
Rights
© 2010 Stramer et al. This article is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
Description
P. Martin and W. Wood contributed equally to this paper
Collections
  • University of St Andrews Research
URL
http://www.scopus.com/inward/record.url?scp=77952335884&partnerID=8YFLogxK
http://jcb.rupress.org/content/189/4/681.abstract
URI
http://hdl.handle.net/10023/2999

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