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Persistent paramyxovirus infections : in co-infections the parainfluenza virus type 5 persistent phenotype is dominant over the lytic phenotype
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dc.contributor.author | Randall, Richard E. | |
dc.contributor.author | Young, Dan F. | |
dc.contributor.author | Hughes, David J. | |
dc.contributor.author | Goodbourn, Steve | |
dc.date.accessioned | 2023-11-17T12:30:01Z | |
dc.date.available | 2023-11-17T12:30:01Z | |
dc.date.issued | 2023-11-14 | |
dc.identifier | 294999394 | |
dc.identifier | ed7c268f-e159-4cfd-8720-e8e9af2d399a | |
dc.identifier | 85176886553 | |
dc.identifier | 85176886553 | |
dc.identifier | 37962188 | |
dc.identifier.citation | Randall , R E , Young , D F , Hughes , D J & Goodbourn , S 2023 , ' Persistent paramyxovirus infections : in co-infections the parainfluenza virus type 5 persistent phenotype is dominant over the lytic phenotype ' , Journal of General Virology , vol. 104 , no. 11 , 001916 . https://doi.org/10.1099/jgv.0.001916 | en |
dc.identifier.issn | 0022-1317 | |
dc.identifier.other | ORCID: /0000-0002-9304-6678/work/146962553 | |
dc.identifier.other | ORCID: /0000-0002-0090-5710/work/146965234 | |
dc.identifier.uri | https://hdl.handle.net/10023/28729 | |
dc.description | Funding: This work was supported by the University of St Andrews and the University of London (St George’s), which are charities registered in Scotland (SC013532 and SC004401, respectively). | en |
dc.description.abstract | Parainfluenza virus type 5 (PIV5) can either have a persistent or a lytic phenotype in cultured cells. We have previously shown that the phenotype is determined by the phosphorylation status of the phosphoprotein (P). Single amino acid substitutions at critical residues, including a serine-to-phenylalanine substitution at position 157 on P, result in a switch between persistent and lytic phenotypes. Here, using PIV5 vectors expressing either mCherry or GFP with persistent or lytic phenotypes, we show that in co-infections the persistent phenotype is dominant. Thus, in contrast to the cell death observed with cells infected solely with the lytic variant, in co-infected cells persistence is immediately established and both lytic and persistent genotypes persist. Furthermore, 10–20 % of virus released from dually infected cells contains both genotypes, indicating that PIV5 particles can package more than one genome. Co-infected cells continue to maintain both genotypes/phenotypes during cell passage, as do individual colonies of cells derived from a culture of persistently infected cells. A refinement of our model on how the dynamics of virus selection may occur in vivo is presented. | |
dc.format.extent | 13 | |
dc.format.extent | 4004533 | |
dc.language.iso | eng | |
dc.relation.ispartof | Journal of General Virology | en |
dc.subject | Paramyxovirus | en |
dc.subject | Persistence | en |
dc.subject | PIV5 | en |
dc.subject | QR355 Virology | en |
dc.subject | Virology | en |
dc.subject | T-NDAS | en |
dc.subject | MCC | en |
dc.subject.lcc | QR355 | en |
dc.title | Persistent paramyxovirus infections : in co-infections the parainfluenza virus type 5 persistent phenotype is dominant over the lytic phenotype | en |
dc.type | Journal article | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.identifier.doi | https://doi.org/10.1099/jgv.0.001916 | |
dc.description.status | Peer reviewed | en |
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