Persistent paramyxovirus infections : in co-infections the parainfluenza virus type 5 persistent phenotype is dominant over the lytic phenotype
Date
14/11/2023Metadata
Show full item recordAbstract
Parainfluenza virus type 5 (PIV5) can either have a persistent or a lytic phenotype in cultured cells. We have previously shown that the phenotype is determined by the phosphorylation status of the phosphoprotein (P). Single amino acid substitutions at critical residues, including a serine-to-phenylalanine substitution at position 157 on P, result in a switch between persistent and lytic phenotypes. Here, using PIV5 vectors expressing either mCherry or GFP with persistent or lytic phenotypes, we show that in co-infections the persistent phenotype is dominant. Thus, in contrast to the cell death observed with cells infected solely with the lytic variant, in co-infected cells persistence is immediately established and both lytic and persistent genotypes persist. Furthermore, 10–20 % of virus released from dually infected cells contains both genotypes, indicating that PIV5 particles can package more than one genome. Co-infected cells continue to maintain both genotypes/phenotypes during cell passage, as do individual colonies of cells derived from a culture of persistently infected cells. A refinement of our model on how the dynamics of virus selection may occur in vivo is presented.
Citation
Randall , R E , Young , D F , Hughes , D J & Goodbourn , S 2023 , ' Persistent paramyxovirus infections : in co-infections the parainfluenza virus type 5 persistent phenotype is dominant over the lytic phenotype ' , Journal of General Virology , vol. 104 , no. 11 , 001916 . https://doi.org/10.1099/jgv.0.001916
Publication
Journal of General Virology
Status
Peer reviewed
ISSN
0022-1317Type
Journal article
Description
Funding: This work was supported by the University of St Andrews and the University of London (St George’s), which are charities registered in Scotland (SC013532 and SC004401, respectively).Collections
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