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dc.contributor.authorRamos-Llorens, Marc
dc.contributor.authorHontoria, Francisco
dc.contributor.authorNavarro, Juan
dc.contributor.authorFerrier, David Ellard Keith
dc.contributor.authorMonroig, Oscar
dc.date.accessioned2023-08-02T11:30:06Z
dc.date.available2023-08-02T11:30:06Z
dc.date.issued2023-10-01
dc.identifier.citationRamos-Llorens , M , Hontoria , F , Navarro , J , Ferrier , D E K & Monroig , O 2023 , ' Functionally diverse front-end desaturases are widespread in the phylum Annelida ' , Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids , vol. 1868 , no. 10 , 159377 . https://doi.org/10.1016/j.bbalip.2023.159377en
dc.identifier.issn1388-1981
dc.identifier.otherPURE: 291319412
dc.identifier.otherPURE UUID: a78095e5-58cd-46a4-8a1c-2d9bf9c53be6
dc.identifier.otherORCID: /0000-0003-3247-6233/work/139964819
dc.identifier.otherScopus: 85166276885
dc.identifier.urihttps://hdl.handle.net/10023/28085
dc.descriptionFunding: This study was funded through the project IMPROMEGA Agencia Española de Investigación, Spain, grant no. RTI2018-095119-B-100, MCIN/AEI/ FEDER/UE / MCIN/AEI/10.13039/501100011033/ and FEDER "A way to make Europe". Additionally, this study forms part of the ThinkInAzul programme and was supported by MCIN with funding from European Union NextGenerationEU (PRTR-C17.I1) and by Generalitat Valenciana (THINKINAZUL/2021/26).en
dc.description.abstractAquatic single-cell organisms have long been believed to be unique primary producers of omega-3 long-chain (≥C20) polyunsaturated fatty acids (ω3 LC-PUFA). Multiple invertebrates including annelids have been discovered to possess methyl-end desaturases enabling key steps in the de novo synthesis of ω3 LC-PUFA, and thus potentially contributing to their production in the ocean. Along methyl-end desaturases, the repertoire and function of further LC-PUFA biosynthesising enzymes is largely missing in Annelida. In this study we examined the front-end desaturase gene repertoire across the phylum Annelida, from Polychaeta and Clitellata, major classes of annelids comprising most annelid diversity. We further characterised the functions of the encoded enzymes in selected representative species by using a heterologous expression system based in yeast, demonstrating that functions of Annelida front-end desaturases have highly diversified during their expansion in both terrestrial and aquatic ecosystems. We concluded that annelids possess at least two front-end desaturases with Δ5 and Δ6Δ8 desaturase regioselectivities, enabling all the desaturation reactions required to convert the C18 precursors into the physiologically relevant LC-PUFA such as eicosapentaenoic and arachidonic acids, but not docosahexaenoic acid. Such a gene complement is conserved across the different taxonomic groups within Annelida.
dc.format.extent10
dc.language.isoeng
dc.relation.ispartofBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipidsen
dc.rightsCopyright © 2023 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/).en
dc.subjectAnnelidaen
dc.subjectBiosynthesisen
dc.subjectFront-end desaturasesen
dc.subjectFunctional diversityen
dc.subjectLong-chain polyunsaturated fatty acidsen
dc.subjectQH301 Biologyen
dc.subjectQH426 Geneticsen
dc.subjectNDASen
dc.subjectMCCen
dc.subject.lccQH301en
dc.subject.lccQH426en
dc.titleFunctionally diverse front-end desaturases are widespread in the phylum Annelidaen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Centre for Biophotonicsen
dc.contributor.institutionUniversity of St Andrews. Scottish Oceans Instituteen
dc.contributor.institutionUniversity of St Andrews. St Andrews Bioinformatics Uniten
dc.contributor.institutionUniversity of St Andrews. Marine Alliance for Science & Technology Scotlanden
dc.identifier.doihttps://doi.org/10.1016/j.bbalip.2023.159377
dc.description.statusPeer revieweden


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