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Investigation of refractive index dynamics during in vitro embryo development using off-axis digital holographic microscopy
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dc.contributor.author | Dwapanyin, George | |
dc.contributor.author | Chow, Darren | |
dc.contributor.author | Tan, Tiffany | |
dc.contributor.author | Dubost, Nicolas | |
dc.contributor.author | Morizet, Josephine | |
dc.contributor.author | Dunning, Kylie | |
dc.contributor.author | Dholakia, Kishan | |
dc.date.accessioned | 2023-07-05T10:30:10Z | |
dc.date.available | 2023-07-05T10:30:10Z | |
dc.date.issued | 2023-07-01 | |
dc.identifier | 287338267 | |
dc.identifier | 02b78a87-cc6b-4cd4-86ec-98c3ec3e0693 | |
dc.identifier | 85166385936 | |
dc.identifier.citation | Dwapanyin , G , Chow , D , Tan , T , Dubost , N , Morizet , J , Dunning , K & Dholakia , K 2023 , ' Investigation of refractive index dynamics during in vitro embryo development using off-axis digital holographic microscopy ' , Biomedical Optics Express , vol. 14 , no. 7 , pp. 3327-3342 . https://doi.org/10.1364/BOE.492292 | en |
dc.identifier.issn | 2156-7085 | |
dc.identifier.uri | https://hdl.handle.net/10023/27891 | |
dc.description | Funding: Engineering and Physical Sciences Research Council - EP/P030017/1, EP/R004854/1; Australian Research Council - FL210100099; National Health and Medical Research Council - APP2003786; Future Making Fellowship (University of Adelaide); Hospital Research Foundation (Mid-career fellowship C-MCF58-2019). | en |
dc.description.abstract | Embryo quality is a crucial factor affecting live birth outcomes. However, an accurate diagnostic for embryo quality remains elusive in the in vitro fertilization clinic. Determining physical parameters of the embryo may offer key information for this purpose. Here, we demonstrate that digital holographic microscopy (DHM) can rapidly and non-invasively assess the refractive index of mouse embryos. Murine embryos were cultured in either low- or high-lipid containing media and digital holograms recorded at various stages of development. The phase of the recorded hologram was numerically retrieved, from which the refractive index of the embryo was calculated. We showed that DHM can detect spatio-temporal changes in refractive index during embryo development that are reflective of its lipid content. As accumulation of intracellular lipid is known to compromise embryo health, DHM may prove beneficial in developing an accurate, non-invasive, multimodal diagnostic. | |
dc.format.extent | 16 | |
dc.format.extent | 7380038 | |
dc.language.iso | eng | |
dc.relation.ispartof | Biomedical Optics Express | en |
dc.subject | QC Physics | en |
dc.subject | QH301 Biology | en |
dc.subject | DAS | en |
dc.subject | MCC | en |
dc.subject.lcc | QC | en |
dc.subject.lcc | QH301 | en |
dc.title | Investigation of refractive index dynamics during in vitro embryo development using off-axis digital holographic microscopy | en |
dc.type | Journal article | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.institution | University of St Andrews. Sir James Mackenzie Institute for Early Diagnosis | en |
dc.contributor.institution | University of St Andrews. Centre for Biophotonics | en |
dc.contributor.institution | University of St Andrews. Institute of Behavioural and Neural Sciences | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.contributor.institution | University of St Andrews. School of Physics and Astronomy | en |
dc.identifier.doi | 10.1364/BOE.492292 | |
dc.description.status | Peer reviewed | en |
dc.identifier.grantnumber | EP/P030017/1 | en |
dc.identifier.grantnumber | EP/R004854/1 | en |
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