Investigation of refractive index dynamics during in vitro embryo development using off-axis digital holographic microscopy
Abstract
Embryo quality is a crucial factor affecting live birth outcomes. However, an accurate diagnostic for embryo quality remains elusive in the in vitro fertilization clinic. Determining physical parameters of the embryo may offer key information for this purpose. Here, we demonstrate that digital holographic microscopy (DHM) can rapidly and non-invasively assess the refractive index of mouse embryos. Murine embryos were cultured in either low- or high-lipid containing media and digital holograms recorded at various stages of development. The phase of the recorded hologram was numerically retrieved, from which the refractive index of the embryo was calculated. We showed that DHM can detect spatio-temporal changes in refractive index during embryo development that are reflective of its lipid content. As accumulation of intracellular lipid is known to compromise embryo health, DHM may prove beneficial in developing an accurate, non-invasive, multimodal diagnostic.
Citation
Dwapanyin , G , Chow , D , Tan , T , Dubost , N , Morizet , J , Dunning , K & Dholakia , K 2023 , ' Investigation of refractive index dynamics during in vitro embryo development using off-axis digital holographic microscopy ' , Biomedical Optics Express , vol. 14 , no. 7 , pp. 3327-3342 . https://doi.org/10.1364/BOE.492292
Publication
Biomedical Optics Express
Status
Peer reviewed
ISSN
2156-7085Type
Journal article
Description
Funding: Engineering and Physical Sciences Research Council - EP/P030017/1, EP/R004854/1; Australian Research Council - FL210100099; National Health and Medical Research Council - APP2003786; Future Making Fellowship (University of Adelaide); Hospital Research Foundation (Mid-career fellowship C-MCF58-2019).Collections
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