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Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry

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dc.contributor.authorDickson, Alison L
dc.contributor.authorYutuc, Eylan
dc.contributor.authorThornton, Catherine A
dc.contributor.authorWang, Yuqin
dc.contributor.authorGriffiths, William J
dc.date.accessioned2023-03-16T14:30:01Z
dc.date.available2023-03-16T14:30:01Z
dc.date.issued2022-11-10
dc.identifier283227497
dc.identifier0be7dff6-d53d-4e7f-9c1b-ac34976ee52f
dc.identifier36440193
dc.identifier85142645042
dc.identifier.citationDickson , A L , Yutuc , E , Thornton , C A , Wang , Y & Griffiths , W J 2022 , ' Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry ' , Frontiers in Endocrinology , vol. 13 , 1031013 . https://doi.org/10.3389/fendo.2022.1031013en
dc.identifier.issn1664-2392
dc.identifier.otherPubMedCentral: PMC9685423
dc.identifier.otherORCID: /0000-0002-4150-2467/work/131123108
dc.identifier.urihttps://hdl.handle.net/10023/27204
dc.descriptionFunding: This work was supported by the Biotechnology and Biological Sciences Research Council (BBSRC, grant numbers BB/I001735/1, BB/N015932/1 and BB/S019588/1 to WG, BB/L001942/1 to YW), the European Union through European Structural Funds (ESF), as part of the Welsh Government funded Academic Expertise for Business project (to WG and YW). AD was supported via a KESS2 award in association with Markes International from the Welsh Government and the European Social Fund.en
dc.description.abstractThe aim of this study was to identify oxysterols and any down-stream metabolites in placenta, umbilical cord blood plasma, maternal plasma and amniotic fluid to enhance our knowledge of the involvement of these molecules in pregnancy. We confirm the identification of 20S-hydroxycholesterol in human placenta, previously reported in a single publication, and propose a pathway from 22R-hydroxycholesterol to a C27 bile acid of probable structure 3β,20R,22R-trihydroxycholest-5-en-(25R)26-oic acid. The pathway is evident not only in placenta, but pathway intermediates are also found in umbilical cord plasma, maternal plasma and amniotic fluid but not non-pregnant women.
dc.format.extent19
dc.format.extent14659915
dc.language.isoeng
dc.relation.ispartofFrontiers in Endocrinologyen
dc.subjectFemaleen
dc.subjectHumansen
dc.subjectPregnancyen
dc.subjectOxysterolsen
dc.subjectChromatography, liquiden
dc.subjectMass Spectrometryen
dc.subjectAmniotic Fluid/metabolismen
dc.subjectFetal Blood/metabolismen
dc.subjectSterolen
dc.subjectCYP11A1en
dc.subjectLC-MSen
dc.subjectCYP27A1en
dc.subjectRC Internal medicineen
dc.subjectRG Gynecology and obstetricsen
dc.subjectNDASen
dc.subjectMCCen
dc.subject.lccRCen
dc.subject.lccRGen
dc.titleIdentification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometryen
dc.typeJournal articleen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.identifier.doi10.3389/fendo.2022.1031013
dc.description.statusPeer revieweden


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