Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry
Abstract
The aim of this study was to identify oxysterols and any down-stream metabolites in placenta, umbilical cord blood plasma, maternal plasma and amniotic fluid to enhance our knowledge of the involvement of these molecules in pregnancy. We confirm the identification of 20S-hydroxycholesterol in human placenta, previously reported in a single publication, and propose a pathway from 22R-hydroxycholesterol to a C27 bile acid of probable structure 3β,20R,22R-trihydroxycholest-5-en-(25R)26-oic acid. The pathway is evident not only in placenta, but pathway intermediates are also found in umbilical cord plasma, maternal plasma and amniotic fluid but not non-pregnant women.
Citation
Dickson , A L , Yutuc , E , Thornton , C A , Wang , Y & Griffiths , W J 2022 , ' Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry ' , Frontiers in Endocrinology , vol. 13 , 1031013 . https://doi.org/10.3389/fendo.2022.1031013
Publication
Frontiers in Endocrinology
Status
Peer reviewed
ISSN
1664-2392Type
Journal article
Description
Funding: This work was supported by the Biotechnology and Biological Sciences Research Council (BBSRC, grant numbers BB/I001735/1, BB/N015932/1 and BB/S019588/1 to WG, BB/L001942/1 to YW), the European Union through European Structural Funds (ESF), as part of the Welsh Government funded Academic Expertise for Business project (to WG and YW). AD was supported via a KESS2 award in association with Markes International from the Welsh Government and the European Social Fund.Collections
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