Show simple item record

Files in this item

Thumbnail

Item metadata

dc.contributor.authorJonaitis, Julius
dc.contributor.authorMacLeod, James
dc.contributor.authorPulver, Stefan R
dc.date.accessioned2022-04-18T17:30:04Z
dc.date.available2022-04-18T17:30:04Z
dc.date.issued2022-04-15
dc.identifier278755539
dc.identifier57fb7c98-3543-42c5-92dd-903a50aa6479
dc.identifier35294308
dc.identifier85128489127
dc.identifier000792347600008
dc.identifier.citationJonaitis , J , MacLeod , J & Pulver , S R 2022 , ' Localization of muscarinic acetylcholine receptor dependent rhythm generating modules in the Drosophila larval locomotor network ' , Journal of Neurophysiology , vol. 127 , no. 4 , pp. 1098-1116 . https://doi.org/10.1152/jn.00106.2021en
dc.identifier.issn0022-3077
dc.identifier.otherJisc: 183522
dc.identifier.otherORCID: /0000-0001-5170-7522/work/111210265
dc.identifier.urihttps://hdl.handle.net/10023/25203
dc.descriptionThis work was supported by the Wellcome Trust through an ISSF award (105621/Z/14/Z) to the University of St Andrews. It was also supported by a Biotechnology and Biological Sciences Research Council (BBSRC) project grant (BB/M021793) awarded to SRP, a CASE studentship awarded to J. M. (BB/M010996/1) and a donation from Kaunas Industrial Water Supply (Kauno Pramoninis Vandentiekis, Kaunas, Lithuania) in support of J. J.en
dc.description.abstractMechanisms of rhythm generation h­­ave been extensively studied in motor systems that control locomotion over terrain in limbed animals; however, much less is known about rhythm generation in soft-bodied terrestrial animals. Here we explored how muscarinic acetylcholine receptor (mAChR) modulated rhythm generating networks are distributed in the central nervous system (CNS) of soft-bodied Drosophila larvae. We measured fictive motor patterns in isolated CNS preparations using a combination of Ca2+ imaging and electrophysiology while manipulating mAChR signalling pharmacologically. Bath application of the mAChR agonist oxotremorine potentiated bilaterally asymmetric activity in anterior thoracic regions and promoted bursting in posterior abdominal regions. Application of the mAChR antagonist scopolamine suppressed rhythm generation in these regions and blocked the effects of oxotremorine. Oxotremorine triggered fictive forward crawling in preparations without brain lobes. Oxotremorine also potentiated rhythmic activity in isolated posterior abdominal CNS segments as well as isolated anterior brain and thoracic regions, but it did not induce rhythmic activity in isolated anterior abdominal segments. Bath application of scopolamine to reduced preparations lowered baseline Ca2+ levels and abolished rhythmic activity. Overall, these results suggest that mAChR signalling plays a role in enabling rhythm generation at multiple sites in the larval CNS. This work furthers our understanding of motor control in soft-bodied locomotion and provides a foundation for study of rhythm generating networks in an emerging genetically tractable locomotor system.
dc.format.extent10619296
dc.language.isoeng
dc.relation.ispartofJournal of Neurophysiologyen
dc.subjectFictive motor patternsen
dc.subjectFruit flyen
dc.subjectCentral Pattern Generatorsen
dc.subjectSoft-bodied locomotionen
dc.subjectMuscarinic receptorsen
dc.subjectQP Physiologyen
dc.subjectNDASen
dc.subject.lccQPen
dc.titleLocalization of muscarinic acetylcholine receptor dependent rhythm generating modules in the Drosophila larval locomotor networken
dc.typeJournal articleen
dc.contributor.sponsorThe Wellcome Trusten
dc.contributor.sponsorBBSRCen
dc.contributor.institutionUniversity of St Andrews. Organic Semiconductor Centreen
dc.contributor.institutionUniversity of St Andrews. School of Psychology and Neuroscienceen
dc.contributor.institutionUniversity of St Andrews. Centre for Biophotonicsen
dc.contributor.institutionUniversity of St Andrews. Institute of Behavioural and Neural Sciencesen
dc.identifier.doihttps://doi.org/10.1152/jn.00106.2021
dc.description.statusPeer revieweden
dc.identifier.grantnumber105621/Z/14/Zen
dc.identifier.grantnumberBB/M021793/1en


This item appears in the following Collection(s)

Show simple item record