St Andrews Research Repository

St Andrews University Home
View Item 
  •   St Andrews Research Repository
  • University of St Andrews Research
  • University of St Andrews Research
  • University of St Andrews Research
  • View Item
  •   St Andrews Research Repository
  • University of St Andrews Research
  • University of St Andrews Research
  • University of St Andrews Research
  • View Item
  •   St Andrews Research Repository
  • University of St Andrews Research
  • University of St Andrews Research
  • University of St Andrews Research
  • View Item
  • Login
JavaScript is disabled for your browser. Some features of this site may not work without it.

Localization of muscarinic acetylcholine receptor dependent rhythm generating modules in the Drosophila larval locomotor network

Thumbnail
View/Open
Jonaitis_2022_JN_Localization_CC.pdf (10.12Mb)
Date
15/04/2022
Author
Jonaitis, Julius
MacLeod, James
Pulver, Stefan R
Funder
The Wellcome Trust
BBSRC
Grant ID
105621/Z/14/Z
BB/M021793/1
Keywords
Fictive motor patterns
Fruit fly
Central Pattern Generators
Soft-bodied locomotion
Muscarinic receptors
QP Physiology
NDAS
Metadata
Show full item record
Altmetrics Handle Statistics
Altmetrics DOI Statistics
Abstract
Mechanisms of rhythm generation h­­ave been extensively studied in motor systems that control locomotion over terrain in limbed animals; however, much less is known about rhythm generation in soft-bodied terrestrial animals. Here we explored how muscarinic acetylcholine receptor (mAChR) modulated rhythm generating networks are distributed in the central nervous system (CNS) of soft-bodied Drosophila larvae. We measured fictive motor patterns in isolated CNS preparations using a combination of Ca2+ imaging and electrophysiology while manipulating mAChR signalling pharmacologically. Bath application of the mAChR agonist oxotremorine potentiated bilaterally asymmetric activity in anterior thoracic regions and promoted bursting in posterior abdominal regions. Application of the mAChR antagonist scopolamine suppressed rhythm generation in these regions and blocked the effects of oxotremorine. Oxotremorine triggered fictive forward crawling in preparations without brain lobes. Oxotremorine also potentiated rhythmic activity in isolated posterior abdominal CNS segments as well as isolated anterior brain and thoracic regions, but it did not induce rhythmic activity in isolated anterior abdominal segments. Bath application of scopolamine to reduced preparations lowered baseline Ca2+ levels and abolished rhythmic activity. Overall, these results suggest that mAChR signalling plays a role in enabling rhythm generation at multiple sites in the larval CNS. This work furthers our understanding of motor control in soft-bodied locomotion and provides a foundation for study of rhythm generating networks in an emerging genetically tractable locomotor system.
Citation
Jonaitis , J , MacLeod , J & Pulver , S R 2022 , ' Localization of muscarinic acetylcholine receptor dependent rhythm generating modules in the Drosophila larval locomotor network ' , Journal of Neurophysiology , vol. 127 , no. 4 , pp. 1098-1116 . https://doi.org/10.1152/jn.00106.2021
Publication
Journal of Neurophysiology
Status
Peer reviewed
DOI
https://doi.org/10.1152/jn.00106.2021
ISSN
0022-3077
Type
Journal article
Rights
Copyright © 2022 The Authors. Open Access article. Licensed under Creative Commons Attribution CC-BY 4.0. Published by the American Physiological Society.
Description
This work was supported by the Wellcome Trust through an ISSF award (105621/Z/14/Z) to the University of St Andrews. It was also supported by a Biotechnology and Biological Sciences Research Council (BBSRC) project grant (BB/M021793) awarded to SRP, a CASE studentship awarded to J. M. (BB/M010996/1) and a donation from Kaunas Industrial Water Supply (Kauno Pramoninis Vandentiekis, Kaunas, Lithuania) in support of J. J.
Collections
  • University of St Andrews Research
URI
http://hdl.handle.net/10023/25203

Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

Advanced Search

Browse

All of RepositoryCommunities & CollectionsBy Issue DateNamesTitlesSubjectsClassificationTypeFunderThis CollectionBy Issue DateNamesTitlesSubjectsClassificationTypeFunder

My Account

Login

Open Access

To find out how you can benefit from open access to research, see our library web pages and Open Access blog. For open access help contact: openaccess@st-andrews.ac.uk.

Accessibility

Read our Accessibility statement.

How to submit research papers

The full text of research papers can be submitted to the repository via Pure, the University's research information system. For help see our guide: How to deposit in Pure.

Electronic thesis deposit

Help with deposit.

Repository help

For repository help contact: Digital-Repository@st-andrews.ac.uk.

Give Feedback

Cookie policy

This site may use cookies. Please see Terms and Conditions.

Usage statistics

COUNTER-compliant statistics on downloads from the repository are available from the IRUS-UK Service. Contact us for information.

© University of St Andrews Library

University of St Andrews is a charity registered in Scotland, No SC013532.

  • Facebook
  • Twitter