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dc.contributor.authorMcHugh, Martin P.
dc.contributor.authorParcell, Benjamin J.
dc.contributor.authorPettigrew, Kerry A.
dc.contributor.authorToner, Geoff
dc.contributor.authorKhatamzas, Elham
dc.contributor.authorel Sakka, Noha
dc.contributor.authorKarcher, Anne Marie
dc.contributor.authorWalker, Joanna
dc.contributor.authorWeir, Robert
dc.contributor.authorMeunier, Danièle
dc.contributor.authorHopkins, Katie L.
dc.contributor.authorWoodford, Neil
dc.contributor.authorTempleton, Kate E.
dc.contributor.authorGillespie, Stephen H.
dc.contributor.authorHolden, Matthew T. G.
dc.identifier.citationMcHugh , M P , Parcell , B J , Pettigrew , K A , Toner , G , Khatamzas , E , el Sakka , N , Karcher , A M , Walker , J , Weir , R , Meunier , D , Hopkins , K L , Woodford , N , Templeton , K E , Gillespie , S H & Holden , M T G 2022 , ' Presence of optrA -mediated linezolid resistance in multiple lineages and plasmids of Enterococcus faecalis revealed by long read sequencing ' , Microbiology , vol. 168 , no. 2 , 001137 .
dc.identifier.otherPURE: 277786295
dc.identifier.otherPURE UUID: cd5a5f7e-eced-41b3-9c90-022da6d49a58
dc.identifier.otherBibtex: mbs:/content/journal/micro/10.1099/mic.0.001137
dc.identifier.otherORCID: /0000-0001-6537-7712/work/108118750
dc.identifier.otherORCID: /0000-0002-4958-2166/work/108118844
dc.identifier.otherORCID: /0000-0002-0370-3700/work/108118900
dc.identifier.otherWOS: 000766808900004
dc.identifier.otherScopus: 85124332213
dc.descriptionFunding: This work was supported by the Chief Scientist Office (Scotland) through the Scottish Healthcare Associated Infection Prevention Institute (Reference SIRN/10). Bioinformatics and Computational Biology analyses were supported by the University of St Andrews Bioinformatics Unit, which is funded by a Wellcome Trust ISSF award [grant 105621/Z/14/Z].en
dc.description.abstractTransferable linezolid resistance due to optrA, poxtA, cfr and cfr-like genes is increasingly detected in enterococci associated with animals and humans globally. We aimed to characterize the genetic environment of optrA in linezolid-resistant Enterococcus faecalis isolates from Scotland. Six linezolid-resistant E. faecalis isolated from urogenital samples were confirmed to carry the optrA gene by PCR. Short read (Illumina) sequencing showed the isolates were genetically distinct (>13900 core SNPs) and belonged to different MLST sequence types. Plasmid contents were examined using hybrid assembly of short and long read (Oxford Nanopore MinION) sequencing technologies. The optrA gene was located on distinct plasmids in each isolate, suggesting that transfer of a single plasmid did not contribute to optrA dissemination in this collection. pTM6294-2, BX5936-1 and pWE0438-1 were similar to optrA-positive plasmids from China and Japan, while the remaining three plasmids had limited similarity to other published examples. We identified the novel Tn6993 transposon in pWE0254-1 carrying linezolid (optrA), macrolide (ermB) and spectinomycin [ANT(9)-Ia] resistance genes. OptrA amino acid sequences differed by 0–20 residues. We report multiple variants of optrA on distinct plasmids in diverse strains of E. faecalis . It is important to identify the selection pressures driving the emergence and maintenance of resistance against linezolid to retain the clinical utility of this antibiotic.
dc.rightsCopyright © 2022 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.en
dc.subjectAntimicrobial resistanceen
dc.subjectEnterococcus faecalisen
dc.subjectQR Microbiologyen
dc.subjectRM Therapeutics. Pharmacologyen
dc.titlePresence of optrA-mediated linezolid resistance in multiple lineages and plasmids of Enterococcus faecalis revealed by long read sequencingen
dc.typeJournal articleen
dc.contributor.sponsorThe Wellcome Trusten
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. Infection and Global Health Divisionen
dc.contributor.institutionUniversity of St Andrews. Sir James Mackenzie Institute for Early Diagnosisen
dc.contributor.institutionUniversity of St Andrews. Centre for Biophotonicsen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews. Global Health Implementation Groupen
dc.contributor.institutionUniversity of St Andrews. Gillespie Groupen
dc.contributor.institutionUniversity of St Andrews. St Andrews Bioinformatics Uniten
dc.description.statusPeer revieweden

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