St Andrews Research Repository

St Andrews University Home
View Item 
  •   St Andrews Research Repository
  • University of St Andrews Research
  • University of St Andrews Research
  • University of St Andrews Research
  • View Item
  •   St Andrews Research Repository
  • University of St Andrews Research
  • University of St Andrews Research
  • University of St Andrews Research
  • View Item
  •   St Andrews Research Repository
  • University of St Andrews Research
  • University of St Andrews Research
  • University of St Andrews Research
  • View Item
  • Login
JavaScript is disabled for your browser. Some features of this site may not work without it.

Presence of optrA-mediated linezolid resistance in multiple lineages and plasmids of Enterococcus faecalis revealed by long read sequencing

Thumbnail
View/Open
McHugh_2022_Presence_of_optrA_mediatied_MIC_001137_CCBY.pdf (806.5Kb)
Date
07/02/2022
Author
McHugh, Martin P.
Parcell, Benjamin J.
Pettigrew, Kerry A.
Toner, Geoff
Khatamzas, Elham
el Sakka, Noha
Karcher, Anne Marie
Walker, Joanna
Weir, Robert
Meunier, Danièle
Hopkins, Katie L.
Woodford, Neil
Templeton, Kate E.
Gillespie, Stephen H.
Holden, Matthew T. G.
Funder
The Wellcome Trust
Grant ID
105621/Z/14/Z
Keywords
Antimicrobial resistance
Enterococcus faecalis
Linezolid
optrA
Plasmid
Tn6993
QR Microbiology
RM Therapeutics. Pharmacology
3rd-DAS
MCC
Metadata
Show full item record
Altmetrics Handle Statistics
Altmetrics DOI Statistics
Abstract
Transferable linezolid resistance due to optrA, poxtA, cfr and cfr-like genes is increasingly detected in enterococci associated with animals and humans globally. We aimed to characterize the genetic environment of optrA in linezolid-resistant Enterococcus faecalis isolates from Scotland. Six linezolid-resistant E. faecalis isolated from urogenital samples were confirmed to carry the optrA gene by PCR. Short read (Illumina) sequencing showed the isolates were genetically distinct (>13900 core SNPs) and belonged to different MLST sequence types. Plasmid contents were examined using hybrid assembly of short and long read (Oxford Nanopore MinION) sequencing technologies. The optrA gene was located on distinct plasmids in each isolate, suggesting that transfer of a single plasmid did not contribute to optrA dissemination in this collection. pTM6294-2, BX5936-1 and pWE0438-1 were similar to optrA-positive plasmids from China and Japan, while the remaining three plasmids had limited similarity to other published examples. We identified the novel Tn6993 transposon in pWE0254-1 carrying linezolid (optrA), macrolide (ermB) and spectinomycin [ANT(9)-Ia] resistance genes. OptrA amino acid sequences differed by 0–20 residues. We report multiple variants of optrA on distinct plasmids in diverse strains of E. faecalis . It is important to identify the selection pressures driving the emergence and maintenance of resistance against linezolid to retain the clinical utility of this antibiotic.
Citation
McHugh , M P , Parcell , B J , Pettigrew , K A , Toner , G , Khatamzas , E , el Sakka , N , Karcher , A M , Walker , J , Weir , R , Meunier , D , Hopkins , K L , Woodford , N , Templeton , K E , Gillespie , S H & Holden , M T G 2022 , ' Presence of optrA -mediated linezolid resistance in multiple lineages and plasmids of Enterococcus faecalis revealed by long read sequencing ' , Microbiology , vol. 168 , no. 2 , 001137 . https://doi.org/10.1099/mic.0.001137
Publication
Microbiology
Status
Peer reviewed
DOI
https://doi.org/10.1099/mic.0.001137
ISSN
1465-2080
Type
Journal article
Rights
Copyright © 2022 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
Description
Funding: This work was supported by the Chief Scientist Office (Scotland) through the Scottish Healthcare Associated Infection Prevention Institute (Reference SIRN/10). Bioinformatics and Computational Biology analyses were supported by the University of St Andrews Bioinformatics Unit, which is funded by a Wellcome Trust ISSF award [grant 105621/Z/14/Z].
Collections
  • University of St Andrews Research
URI
http://hdl.handle.net/10023/24819

Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

Advanced Search

Browse

All of RepositoryCommunities & CollectionsBy Issue DateNamesTitlesSubjectsClassificationTypeFunderThis CollectionBy Issue DateNamesTitlesSubjectsClassificationTypeFunder

My Account

Login

Open Access

To find out how you can benefit from open access to research, see our library web pages and Open Access blog. For open access help contact: openaccess@st-andrews.ac.uk.

Accessibility

Read our Accessibility statement.

How to submit research papers

The full text of research papers can be submitted to the repository via Pure, the University's research information system. For help see our guide: How to deposit in Pure.

Electronic thesis deposit

Help with deposit.

Repository help

For repository help contact: Digital-Repository@st-andrews.ac.uk.

Give Feedback

Cookie policy

This site may use cookies. Please see Terms and Conditions.

Usage statistics

COUNTER-compliant statistics on downloads from the repository are available from the IRUS-UK Service. Contact us for information.

© University of St Andrews Library

University of St Andrews is a charity registered in Scotland, No SC013532.

  • Facebook
  • Twitter