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Linescan microscopy data to extract diffusion coefficient of a fluorescent species using a commercial confocal microscope
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dc.contributor.author | Bathe-Peters, Marc | |
dc.contributor.author | Gmach, Philipp | |
dc.contributor.author | Annibale, Paolo | |
dc.contributor.author | Lohse, Martin J | |
dc.date.accessioned | 2022-01-17T16:30:19Z | |
dc.date.available | 2022-01-17T16:30:19Z | |
dc.date.issued | 2020-04 | |
dc.identifier.citation | Bathe-Peters , M , Gmach , P , Annibale , P & Lohse , M J 2020 , ' Linescan microscopy data to extract diffusion coefficient of a fluorescent species using a commercial confocal microscope ' , Data in Brief , vol. 29 , 105063 . https://doi.org/10.1016/j.dib.2019.105063 | en |
dc.identifier.issn | 2352-3409 | |
dc.identifier.other | PURE: 277343382 | |
dc.identifier.other | PURE UUID: 886f226e-cb64-452d-8c2e-7456784ebbff | |
dc.identifier.other | PubMed: 32055652 | |
dc.identifier.other | PubMedCentral: PMC7005367 | |
dc.identifier.other | Scopus: 85077921706 | |
dc.identifier.other | ORCID: /0000-0003-3208-5347/work/105957254 | |
dc.identifier.uri | https://hdl.handle.net/10023/24686 | |
dc.description | We are grateful to the Max Delbrück Center for Molecular Medicine in the Helmholtz Association for core support and funding. P.A. and M.J.L. would like to acknowledge funding from the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – Project-ID 421152132-SFB1423 subproject C03. | en |
dc.description.abstract | We report here on the measurement of the diffusion coefficient of fluorescent species using a commercial microscope possessing a resonant scanner. Sequential linescans with a rate of up to 12 kHz yield a temporal resolution of 83 μs, making the setup amenable to measure diffusion rates over a range covering at least three orders of magnitude, from 100 μm2/s down to 0.1 μm2/s. We share representative data sets covering (i) the diffusion of a dye molecule, observed in media of different viscosities and (ii) the diffusion of a prototypical membrane receptor. The data can be valuable for researchers interested in the rapid diffusion properties of nuclear, cytosolic or membrane bound proteins fused to fluorescent tags. | |
dc.format.extent | 5 | |
dc.language.iso | eng | |
dc.relation.ispartof | Data in Brief | en |
dc.rights | Copyright © 2020 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons. org/licenses/by/4.0/). | en |
dc.subject | Confocal microscopy | en |
dc.subject | Fluorescence correlatioon spectroscopy | en |
dc.subject | Member receptor | en |
dc.subject | Diffusion | en |
dc.subject | QC Physics | en |
dc.subject | QH301 Biology | en |
dc.subject | NDAS | en |
dc.subject.lcc | QC | en |
dc.subject.lcc | QH301 | en |
dc.title | Linescan microscopy data to extract diffusion coefficient of a fluorescent species using a commercial confocal microscope | en |
dc.type | Journal article | en |
dc.description.version | Publisher PDF | en |
dc.contributor.institution | University of St Andrews. School of Physics and Astronomy | en |
dc.identifier.doi | https://doi.org/10.1016/j.dib.2019.105063 | |
dc.description.status | Peer reviewed | en |
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