Linescan microscopy data to extract diffusion coefficient of a fluorescent species using a commercial confocal microscope
Abstract
We report here on the measurement of the diffusion coefficient of fluorescent species using a commercial microscope possessing a resonant scanner. Sequential linescans with a rate of up to 12 kHz yield a temporal resolution of 83 μs, making the setup amenable to measure diffusion rates over a range covering at least three orders of magnitude, from 100 μm2/s down to 0.1 μm2/s. We share representative data sets covering (i) the diffusion of a dye molecule, observed in media of different viscosities and (ii) the diffusion of a prototypical membrane receptor. The data can be valuable for researchers interested in the rapid diffusion properties of nuclear, cytosolic or membrane bound proteins fused to fluorescent tags.
Citation
Bathe-Peters , M , Gmach , P , Annibale , P & Lohse , M J 2020 , ' Linescan microscopy data to extract diffusion coefficient of a fluorescent species using a commercial confocal microscope ' , Data in Brief , vol. 29 , 105063 . https://doi.org/10.1016/j.dib.2019.105063
Publication
Data in Brief
Status
Peer reviewed
ISSN
2352-3409Type
Journal article
Rights
Copyright © 2020 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons. org/licenses/by/4.0/).
Description
We are grateful to the Max Delbrück Center for Molecular Medicine in the Helmholtz Association for core support and funding. P.A. and M.J.L. would like to acknowledge funding from the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – Project-ID 421152132-SFB1423 subproject C03.Collections
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