Quantitative spectroscopy of single molecule interaction times
Date
01/04/2021Metadata
Show full item recordAbstract
Single molecule fluorescence tracking provides information at nanometer-scale and millisecond-temporal resolution about the dynamics and interaction of individual molecules in a biological environment. While the dynamic behavior of isolated molecules can be characterized well, the quantitative insight is more limited when interactions between two indistinguishable molecules occur. We address this aspect by developing a theoretical foundation for a spectroscopy of interaction times, i.e., the inference of interaction from imaging data. A non-trivial crossover between a power law to an exponential behavior of the distribution of the interaction times is highlighted, together with the dependence of the exponential term upon the microscopic reaction affinity. Our approach is validated with simulated and experimental datasets.
Citation
Boltz , H H , Sirbu , A , Stelzer , N , Lohse , M J , Schütte , C & Annibale , P 2021 , ' Quantitative spectroscopy of single molecule interaction times ' , Optics Letters , vol. 46 , no. 7 , pp. 1538-1541 . https://doi.org/10.1364/OL.413030
Publication
Optics Letters
Status
Peer reviewed
ISSN
0146-9592Type
Journal article
Description
Funding Information: Deutsche Forschungsgemeinschaft (421152132 (subproject C03)), EXC 2046 (MATH+Incubator project IN-B2).Collections
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