Shining new light on sensory brain activation and physiological measurement in seals using wearable optical technology

Abstract

Sensory ecology and physiology of free-ranging animals is challenging to study but underpins our understanding of decision making in the wild. Existing non-invasive human biomedical technology offers tools that could be harnessed to address these challenges. Functional near-infrared spectroscopy (fNIRS), a wearable, non-invasive biomedical imaging technique measures oxy- and deoxyhemoglobin concentration changes that can be used to detect localised neural activation in the brain. We tested the efficacy of fNIRS to detect cortical activation in grey seals (Halichoerus grypus) and identify regions of the cortex associated with different senses (vision, hearing and touch). Activation of specific cerebral areas in seals was detected by fNIRS in responses to light (vision), sound (hearing) and whisker stimulation (touch). Physiological parameters, including heart and breathing rate, were also extracted from the fNIRS signal, which allowed neural and physiological responses to be monitored simultaneously. This is the first time fNIRS has been used to detect cortical activation in a non-domesticated or laboratory animal. Since fNIRS is non-invasive and wearable, this study demonstrates its potential as a tool to quantitatively investigate sensory perception and brain function while simultaneously recording physiological dynamics that allow calculation of heart rate, tissue and arterial oxygen saturation of haemoglobin, respectively, perfusion changes and breathing rate in free-ranging animals.