Regulation of coordinated muscular relaxation in Drosophila larvae by a pattern-regulating intersegmental circuit
MetadataShow full item record
Typical patterned movements in animals are achieved through combinations of contraction and delayed relaxation of groups of muscles. However, how intersegmentally coordinated patterns of muscular relaxation are regulated by the neural circuits remains poorly understood. Here, we identify Canon, a class of higher-order premotor interneurons, that regulates muscular relaxation during backward locomotion of Drosophila larvae. Canon neurons are cholinergic interneurons present in each abdominal neuromere and show wave-like activity during fictive backward locomotion. Optogenetic activation of Canon neurons induces relaxation of body wall muscles, whereas inhibition of these neurons disrupts timely muscle relaxation. Canon neurons provide excitatory outputs to inhibitory premotor interneurons. Canon neurons also connect with each other to form an intersegmental circuit and regulate their own wave-like activities. Thus, our results demonstrate how coordinated muscle relaxation can be realized by an intersegmental circuit that regulates its own patterned activity and sequentially terminates motor activities along the anterior-posterior axis.
Hiramoto , A , Jonaitis , J , Niki , S , Kohsaka , H , Fetter , R D , Cardona , A , Pulver , S R & Nose , A 2021 , ' Regulation of coordinated muscular relaxation in Drosophila larvae by a pattern-regulating intersegmental circuit ' , Nature Communications , vol. 12 , 2943 . https://doi.org/10.1038/s41467-021-23273-y
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
DescriptionThis work was supported by HHMI Janelia Visitor program (A.H. and S.R.P. hosted by A.C.), the Howard Hughes Medical Institute (R.D.F. and A.C.), Wellcome Trust Institutional Strategic Support Funds (105621/Z/14/Z) (S.R.P.), a Royal Society Research Grant (RG150108) (S.R.P.), and MEXT/JSPS KAKENHI grants (20K06908 and 17K07042 to H.K., 15H04255, 17H05554, 18H05113, 19H04742, and 20H05048 to A.N.).
Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.