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Molecular detection of mycobacterium tuberculosis in oral mucosa from patients with presumptive tuberculosis

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Molina_Moya_2020_Molecular_detection_JCM_09_04124.pdf (209.8Kb)
Date
21/12/2020
Author
Molina-Moya, Barbara
Ciobanu, Nelly
Hernandez, Marta
Prat-Aymerich, Cristina
Crudu, Valeriu
Adams, Emily R.
Codreanu, Alexandru
Sloan, Derek J.
Cuevas, Luis E.
Dominguez, Jose
Keywords
Oral swab
Diagnosis
Real-time PCR
QR Microbiology
RC Internal medicine
E-NDAS
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Abstract
Tuberculosis (TB) diagnosis is increasingly based on the detection of Mycobacterium tuberculosis complex (MTBC) DNA in sputum using molecular diagnostic tests as the first test for diagnosis. However, sputum can be difficult to obtain in children, patients without productive cough, and the elderly and approaches testing non-sputum samples are needed. We evaluated whether TB can be detected from the oral mucosa of patients with TB. Adults with presumptive TB were examined using culture, Xpert MTB/RIF, smear microscopy and X-Rays. Oral mucosa swabs collected on PrimeStore-MTM, stored at room temperature if tested within 30 days or at −20 °C if examined at a later time. RT-PCR was performed to detect M. tuberculosis DNA. Eighty patients had bacteriologically-confirmed TB, 34 had bacteriologically-negative TB (negative tests but abnormal X-rays) and 152 were considered not to have TB (not TB). Oral swabs RT-PCR were positive in 29/80 (36.3%) bacteriologically-confirmed, 9/34 (26.5%) bacteriologically-negative and 29/152 (19.1%) not TB. The yield varied among samples stored for less and more than 30 days (p = 0.013) from 61% (11/18) and 29% (18/62) among bacteriologically confirmed, and 30.8% (4/13) and 23.8% (5/21) among bacteriologically-negative participants. Among not TB patients, the specificity was 80.9% (123/152), being 78.3% (18/23) among samples stored less than 30 days and 81.4% (105/129) among samples stored for more than 30 days (p = 0.46). The detection of M. tuberculosis in oral mucosa samples is feasible, but storage conditions may affect the yield.
Citation
Molina-Moya , B , Ciobanu , N , Hernandez , M , Prat-Aymerich , C , Crudu , V , Adams , E R , Codreanu , A , Sloan , D J , Cuevas , L E & Dominguez , J 2020 , ' Molecular detection of mycobacterium tuberculosis in oral mucosa from patients with presumptive tuberculosis ' , Journal of Clinical Medicine , vol. 9 , no. 12 , 4124 . https://doi.org/10.3390/jcm9124124
Publication
Journal of Clinical Medicine
Status
Peer reviewed
DOI
https://doi.org/10.3390/jcm9124124
ISSN
2077-0383
Type
Journal article
Rights
Copyright ©2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution(CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Description
Funding: This research was funded by a Strategic Award grant from the European and Developing Countries Clinical Trials Partnership (grant DRIA2014-309) and its cofounders, the Medical Research Council UK, and Institutode Salud Carlos III (ISCIII), Spain (PI116/01912); and from the European Union’s Horizon 2020 Research and Innovation Programme under the Marie Skłodowska-Curie grant agreement no. 823854 (INNOVA4TB).
Collections
  • University of St Andrews Research
URI
http://hdl.handle.net/10023/21183

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